表面修饰的纳米粒子点击进入纳米粒子簇以进行寡核苷酸封装,RSC Advances

当前位置： X-MOL 学术 › RSC Adv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

表面修饰的纳米粒子点击进入纳米粒子簇以进行寡核苷酸封装
RSC Advances ( IF 3.9 ) Pub Date : 2020-10-7 , DOI: 10.1039/d0ra06622b
Wei Mao ₁ , Song Rae Kim ₂ , Hyuk Sang Yoo _{1,

3}

Affiliation

金纳米粒子 (AuNPs) 是用于肿瘤靶向递送治疗剂的主要和代表性金属纳米载体，因为它们具有生物相容性、高载药效率以及通过尺寸依赖性增强渗透性和增强在肿瘤部位的积累等优点。保留（EPR）效应。在这项研究中，我们设计了一种用包含聚乙烯亚胺和叠氮基官能化聚（乙二醇）的嵌段聚合物功能化的 AuNP，用于在表面上静电掺入胞嘧啶 - 鸟嘌呤寡核苷酸（CpG ODN）。结合 ODN 的 AuNPs 通过交联到金纳米粒子簇 (AuNCs )click 化学使用在两端用炔基修饰的基质金属蛋白酶 (MMP)-2 可切割肽接头。在存在 Cu( I ) 的情况下，叠氮基和炔基自发环化以形成具有高保真度和效率的三唑环，因此允许单个 AuNPs 堆叠到更大的 AuNCs 以增加 EPR 效应介导的肿瘤靶向性。¹H-NMR和傅里叶变换红外光谱揭示了叠氮化物-PEG接枝的支化聚乙烯亚胺的成功合成，并且通过场发射透射电子显微镜证实由合成聚合物制备的AuNPs的尺寸和形态为4.02±0.45 nm。拉曼光谱表征表明在合成的 AuNPs 表面上引入了叠氮基团。负载 CpG 的 AuNPs 的 Zeta 电位和凝胶延迟分析表明，当 CpG:AuNP 重量比高于 1:2.5 时，AuNPs 完全隔离 CpG。对负载 CpG 的 AuNPs 的聚类过程进行了监测，并证明这取决于炔烃接头与 AuNP 的比率。因此，可以将点击的 AuNC 定制为需要大量治疗药物的基因载体。

"点击查看英文标题和摘要"

Surface-decorated nanoparticles clicked into nanoparticle clusters for oligonucleotide encapsulation

Gold nanoparticles (AuNPs) are the predominant and representative metal nano-carriers used for the tumor-targeted delivery of therapeutics because they possess advantages such as biocompatibility, high drug loading efficiency, and enhanced accumulation at tumor sites via the size-dependent enhanced permeability and retention (EPR) effect. In this study, we designed an AuNP functionalized with block polymers comprising polyethylenimine and azide group-functionalized poly(ethyl glycol) for the electrostatic incorporation of cytosine–guanine oligonucleotide (CpG ODN) on the surface. The ODN-incorporated AuNPs were cross-linked to gold nanoparticle clusters (AuNCs) via click chemistry using a matrix metalloproteinase (MMP)-2 cleavable peptide linker modified with alkyne groups at both ends. In the presence of Cu(I), azide groups and alkyne groups spontaneously cyclize to form a triazole ring with high fidelity and efficiency, and therefore allow single AuNPs to stack to larger AuNCs for increased EPR effect-mediated tumor targeting. ¹H-NMR and Fourier transform infrared spectroscopy revealed the successful synthesis of an azide–PEG-grafted branched polyethylenimine, and the size and morphology of AuNPs fabricated by the synthesized polymer were confirmed to be 4.02 ± 0.45 nm by field emission-transmission electron microscopy. Raman spectroscopy characterization demonstrated the introduction of azide groups on the surface of the synthesized AuNPs. Zeta-potential and gel-retardation analysis of CpG-loaded AuNPs indicated complete CpG sequestration by AuNPs when the CpG : AuNP weight ratio was higher than 1 : 2.5. The clustering process of the CpG-loaded AuNPs was monitored and was demonstrated to be dependent on the alkyne linker-to-AuNP ratio. Thus, the clicked AuNC can be tailored as a gene carrier where a high accumulation of therapeutics is required.

更新日期：2020-10-07

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南