Bm30kc6基因在家蚕细胞凋亡和丝腺降解信号通路中的作用,Archives of Insect Biochemistry and Physiology

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Bm30kc6基因在家蚕细胞凋亡和丝腺降解信号通路中的作用
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-10-01 , DOI: 10.1002/arch.21741
Ying Xiao ₁ , Lei‐lei Li ₁ , Asma Bibi ₁ , Ning Zhang ₂ , Ting Chen ₁ , Yitao Mo ₁ , Wanfu Yue ₂ , Yungen Miao ₁

Affiliation

凋亡是程序性细胞死亡的过程，其由基因独立调节。该 Bm30kc6基因是一种约30 kDa的小分子脂蛋白，在家蚕血液后期高度表达。我们的研究表明，Bm30kc6的过表达可以降低caspase-3的激活。同时，当Bm30kc6表达受小干扰RNA（siRNA）干扰时，caspase-3的激活增加。Bm30kc6在紫外线处理下过表达时，细胞凋亡减少。放线菌素D诱导的凋亡率与紫外线的趋势相似。推测Bm30kc6对蚕细胞的凋亡具有抑制作用。凋亡相关基因，例如BmFadd，BmDredd和BmDaxx被过度表达后增加Bm30kc6 或siRNA的干扰后下降。据推测，之间存在着一种互动的关系Bm30kc6，BmDaxx，BmFadd和BmDredd在细胞凋亡信号通路。我们调查了Bm30kc6基因在家蚕不同生长阶段和组织中的转录表达。结果表明，Bm30kc6在第5龄幼虫的第6至第7天，或在丝腺24小时后的纺丝过程中，在淋巴中达到最高峰。在与caspase-3/7抑制剂，胱天蛋白酶-3酶的活性，表达水平处理的蚕家蚕细胞Bm30kc6，BmFadd，BmDredd和BmDaxx均显著减少。在第5龄第3天或第5天用蜕皮激素处理家蚕后，Bm30kc6的表达水平急剧增加。结果表明，Bm30kc6基因的表达受蜕皮激素的影响，很可能是其下游靶标。总之，结果表明 Bm30kc6 基因参与凋亡信号通路的调节，并在凋亡过程中发挥作用。

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Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

Apoptosis is a process of programmed cell death that is regulated by genes independently. The Bm30kc6 gene is a kind of small molecular lipoprotein about 30 kDa, expressed highly in the late stage of the silkworm hemolymph. Our study showed that overexpression of Bm30kc6 could decrease caspase‐3 activation. Meanwhile, activation of caspase‐3 increased when Bm30kc6 expression was disturbed by small interfering RNA (siRNA). Cell apoptosis was decreased when Bm30kc6 was overexpressed under UV treatment. The apoptosis rate induced by actinomycin D is similar to the trend by UV. It was inferred that Bm30kc6 has an inhibitory effect on the apoptosis of silkworm cells. The apoptosis‐related genes, such as BmFadd, BmDredd, and BmDaxx were increased after overexpression of Bm30kc6 or decreased after interference of siRNA. It was speculated that there was an interactive relationship between Bm30kc6, BmDaxx, BmFadd, and BmDredd in the apoptosis signaling pathways. We investigated the transcription expression of the Bm30kc6 gene in different growth stages and tissues of the silkworm. The results showed that Bm30kc6 reached its peak in the hemolymph during the 6th to 7th days of the 5th instar, or in spinning post 24 h of the silk gland. In the silkworm BmN cells treated with caspase‐3/7 inhibitor, the caspase‐3 enzyme activity, and the expression levels of Bm30kc6, BmFadd, BmDredd, and BmDaxx were significantly reduced. The expression levels of Bm30kc6 increased sharply when silkworms were treated by molting hormone at Day 3 or 5 of the 5th instar. The results indicated that the expression of the Bm30kc6 gene was affected by the molting hormone and was likely to be its downstream target. In conclusion, the results suggest that the Bm30kc6 gene is involved in the regulation of the apoptotic signaling pathway and plays a role in the apoptotic process.

更新日期：2020-10-12

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