Microfluidic electrochemical immunosensor for the determination of cystatin C in human serum,Microchimica Acta

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Microfluidic electrochemical immunosensor for the determination of cystatin C in human serum
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-30 , DOI: 10.1007/s00604-020-04503-4
K S Shalini Devi _{1,

2} , Uma Maheswari Krishnan _{1,

2,

3}

Affiliation

The fabrication of a nanointerfaced electrochemical immunosensor is described for the rapid determination of cystatin C, a biomarker that is elevated in diabetic retinopathy. A dispersion of graphene oxide-chitosan (GO-Chit) nanocomposite was used to modify the carbon working electrode, allowing for a high conjugation of anti–cystatin C antibody. This modified sensor was characterized both morphologically and electrochemically, and the sensor performance was evaluated towards selective quantification of cystatin C in simulated as well as serum samples using cyclic voltammetry and differential pulse voltammetry. The sensor was able to detect cystatin C in the concentration range1 - 10 mg/L with a detection limit of 0.0078 mg/L. The preparation time of the sensor was 420 s, which was faster than that of conventional ELISA and other electrochemical sensors reported in literature. The clinical applicability of the proposed electrochemical biosensor was demonstrated through quantification of cystatin C in human serum samples and identification of diabetic retinopathy. A cutoff value of 1.2 mg/L of cystatin C was used beyond which the samples were classified as positive for diabetic retinopathy. Two different working electrodes, namely a glassy carbon electrode (GCE) and paper electrodes, were used in the study. The working potential was set to 0.25 V vs. Ag/AgCl for experiments with the GCE and 0.15 V for the paper electrodes. The prediction was validated by clinical diagnosis wherein the prediction accuracy of the sensor exceeded 85%. The sensor platform was translated onto a paper substrate and characterized for achieving an optimum sensing performance. This work is the first attempt to employ an electrochemical cystatin C sensor for the diagnosis of diabetic retinopathy from serum samples. Graphical abstract Graphical abstract

中文翻译：

用于测定人血清中胱抑素C的微流体电化学免疫传感器

描述了纳米界面电化学免疫传感器的制造，用于快速测定胱抑素 C，这是一种在糖尿病视网膜病变中升高的生物标志物。氧化石墨烯-壳聚糖 (GO-Chit) 纳米复合材料的分散体用于修饰碳工作电极，从而实现抗胱抑素 C 抗体的高度结合。这种改良的传感器在形态学和电化学上都进行了表征，并且使用循环伏安法和差分脉冲伏安法对模拟和血清样品中胱抑素 C 的选择性定量评估了传感器的性能。该传感器能够检测浓度范围为 1 - 10 mg/L 的胱抑素 C，检测限为 0.0078 mg/L。传感器的准备时间为 420 s，这比文献中报道的传统 ELISA 和其他电化学传感器更快。所提出的电化学生物传感器的临床适用性通过人血清样品中胱抑素 C 的定量和糖尿病视网膜病变的鉴定得到证明。使用 1.2 mg/L 的半胱氨酸蛋白酶抑制剂 C 的临界值，超过该临界值，样品被归类为糖尿病视网膜病变阳性。研究中使用了两种不同的工作电极，即玻璃碳电极 (GCE) 和纸电极。对于 GCE 实验，工作电位设置为 0.25 V vs. Ag/AgCl，对于纸电极设置为 0.15 V。该预测通过临床诊断得到验证，其中传感器的预测准确度超过 85%。传感器平台被转移到纸基板上，并表征为实现最佳传感性能。这项工作是首次尝试使用电化学胱抑素 C 传感器从血清样本诊断糖尿病视网膜病变。图形摘要图形摘要

更新日期：2020-09-30

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