Enhancing Photostability of a Coumarin Dye by Self‐inclusion into a Cyclodextrin Cavity in Aqueous Solution and Living Cells,Asian Journal of Organic Chemistry

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Enhancing Photostability of a Coumarin Dye by Self‐inclusion into a Cyclodextrin Cavity in Aqueous Solution and Living Cells
Asian Journal of Organic Chemistry ( IF 2.8 ) Pub Date : 2020-09-28 , DOI: 10.1002/ajoc.202000365
Hajime Shigemitsu ₁ , Keigo Matsuda ₂ , Tadashi Mori ₂ , Hirotaka Nakatsuji ₂ , Michiya Matsusaki ₂ , Toshiyuki Kida ₂

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Photostable organic dyes are highly demanded for longtime or super‐resolution bioimaging. Herein, we demonstrate effective improvement of photostability of a coumarin dye (Pacific Blue (PB)) by steric protection using a cyclodextrin. The PB conjugated cyclodextrin (PB−CD) showed 2.8 times higher photostability than PB ethyl amide (PB−EA) which is a comparative compound in vitro. The cyclodextrin conjugation to PB not only protect PB unit from reactive chemicals, but also suppress reactive oxygen species (ROS) generation which causes dye degradation. The effects afford significant improvement of photostability of PB dye. Finally, the photostability of PB−CD was evaluated using confocal laser scanning microscopy (CLSM) and the significant improvement effect was proofed even in living cells.

中文翻译：

通过自掺入水溶液和活细胞中的环糊精腔中来增强香豆素染料的光稳定性

长期或超分辨率生物成像对光稳定的有机染料有很高的要求。在本文中，我们证明了通过使用环糊精进行空间保护可以有效提高香豆素染料（太平洋蓝（PB））的光稳定性。所述PB缀合的环糊精（PB-CD）显示出更高的2.8倍的光稳定性比PB乙基酰胺（PB-EA），其是在体外比较化合物。环糊精与PB的结合不仅可以保护PB单元不受活性化学物质的影响，而且还可以抑制导致染料降解的活性氧（ROS）的产生。该效果显着改善了PB染料的光稳定性。最后，光稳定性使用共聚焦激光扫描显微镜（CLSM）评估了PB-CD，即使在活细胞中也证明了显着的改善效果。

更新日期：2020-09-28

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