In this work, LiYF₄:Yb_0.25³⁺/Er_0.01³⁺/Tm_0.01³⁺/Ho_0.01³⁺@LiYF₄:Yb_0.2³⁺ upconverting nanoparticles (UCNP) were used as luminescent materials for the preparation of molecular imprinting polymer nanocomposites. Three luminescent molecularly imprinted polymer (MIP) nanocomposites were prepared by in situ polymerization. The relationship between the functional monomers, templates, and upconversion nanoparticles was investigated. Two hydrophilic monomers (acrylic acid (AA) and acrylamide (AAm)) and one hydrophobic monomer (N-tert-butylacrylamide (TBAm)) were employed as functional monomers, while one amino acid (cysteine) and two proteins (albumin and hemoglobin) were employed as the templates to investigate the effect of their interaction with LiYF₄:Yb³⁺/Er³⁺/Ho³⁺/Tm³⁺@LiYF₄:Yb³⁺ core/shell UCNPs on the polymerization process, luminescence properties, and adsorption capacity. The results showed that the UCNPs were embedded in the polymeric matrix to form an irregular quasimicrospherical UCNPs@MIP with diameters ranging from several hundred nanometers to several micrometers depending on the functional monomer. The quenching effect was more pronounced for the adsorption of hemoglobin with UCNPs@MIP compared to cysteine and albumin. In addition, the adsorption capacities of the AA- and AAm-made UCNPs@MIP were greater than those of TBAm-made UCNPs@MIP. The rebinding of the templates onto UCNPs@MIP was very fast and approached equilibrium within 30 min, indicating that the synthesized UCNPs@MIP can be employed as fluorescent probes to offer rapid detection of molecules.

中文翻译：

---

LiYF4:Yb3+/Er3+/Ho3+/Tm3+@LiYF4:Yb3+上转换纳米粒子、分子印迹聚合物和模板的相互作用

本工作以LiYF ₄ :Yb _0.25 ³⁺ /Er _0.01 ³⁺ /Tm _0.01 ³⁺ /Ho _0.01 ³⁺ @LiYF ₄ :Yb _0.2 ³⁺上转换纳米粒子（UCNP）作为发光材料制备分子印迹聚合物纳米复合材料。通过原位聚合制备了三种发光分子印迹聚合物（MIP）纳米复合材料。研究了功能单体、模板和上转换纳米粒子之间的关系。两种亲水单体（丙烯酸（AA）和丙烯酰胺（AAm））和一种疏水单体（N-叔-丁基丙烯酰胺（TBAm）作为功能单体，而一种氨基酸（半胱氨酸）和两种蛋白质（白蛋白和血红蛋白）作为模板来研究它们与LiYF ₄ :Yb ³⁺ /Er ³⁺相互作用的影响/Ho ³⁺ /Tm ³⁺ @LiYF ₄ :Yb ³⁺核/壳 UCNPs 对聚合过程、发光特性和吸附能力的影响。结果表明，UCNPs嵌入聚合物基质中，形成不规则的准微球状UCNPs@MIP，其直径取决于功能单体，直径从几百纳米到几微米不等。与半胱氨酸和白蛋白相比，UCNPs@MIP 对血红蛋白的吸附猝灭效应更为明显。此外，AA 和 AAm 制造的 UCNPs@MIP 的吸附能力大于 TBAm 制造的 UCNPs@MIP。模板在 UCNPs@MIP 上的重新结合非常快，并在 30 分钟内达到平衡，表明合成的 UCNPs@MIP 可用作荧光探针以提供分子的快速检测。