A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing,Chemical Communications

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A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing
Chemical Communications ( IF 4.3 ) Pub Date : 2020-09-28 , DOI: 10.1039/d0cc06034h
Weimin Tian _{1,

2,

3,

4,

5} , Xiaoling Liu _{1,

2,

3,

4,

5} , Gaoting Wang _{1,

2,

3,

4,

5} , Chenghui Liu _{1,

2,

3,

4,

5}

Affiliation

A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.

中文翻译：

超分支转录激活的CRISPR-Cas12a信号放大策略，用于敏感的microRNA传感

开发了一种基于CRISPR-Cas12a的策略，用于敏感的microRNA感应，其中整合了超支化滚环扩增和转录，以激活Cas12a的反式切割能力。集成的三路信号放大使该策略具有低背景和高灵敏度。这种设计扩展了基于CRISPR-Cas的传感工具箱的范围，并在生物学和生物医学研究中显示出巨大的潜力。

更新日期：2020-10-12

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