Verticillium wilt caused by Verticillium dahliae Kleb. is a major disease in cotton. We found that pectin lyase can enhance cotton resistance to Verticillium wilt and induce cell apoptosis of V. dahliae strain Vd080. The biocontrol effect of pectin lyase on Vd080 reached 61.9%. Pectin lyase increased ERG4 (Delta (24 (24 (1)))-sterol reductase) expression, the ergosterol content of the cell membrane, the collapse of mitochondrial membrane potential, hydrogen peroxide content, metacaspase activity, and Ca²⁺ content in the cytoplasm in the Vd080 strain and induced endoplasmic reticulum (ER) stress. Pectin lyase also increased the expression levels of the ER molecular chaperone glucose regulating protein Grp78 (BiP), protein disulfide isomerase (PDI) and calnexin (CNX), reduced the expression levels of the protein Hsp40. When the PDI and BiP genes of Vd080 were knocked out, the mutants △BiP and △PDI had reduced sensitivity to pectin lyase. In the absence of external stress, ER stress appeared in mutant △BiP cells. Pectin lyase affects the ergosterol content of the Vd080 cell membrane, which causes ER stress and increases the level of BiP to induce Vd080 cell apoptosis. These results demonstrate that pectin lyase can be used to control Verticillium wilt in cotton.

由大丽花黄萎病菌引起的黄萎病。是棉花的主要病害。我们发现果胶裂解酶可以增强棉花对黄萎病的抗性并诱导大丽花菌株Vd080的细胞凋亡。果胶裂解酶对Vd080的生防效果达到61.9%。果胶裂解酶增加 ERG4 (Delta (24 (24 (1)))-sterol reductase) 表达、细胞膜的麦角甾醇含量、线粒体膜电位塌陷、过氧化氢含量、间半胱天冬酶活性和 Ca ²⁺Vd080 菌株细胞质中的含量和诱导的内质网 (ER) 应激。果胶裂解酶还增加了 ER 分子伴侣葡萄糖调节蛋白 Grp78 (BiP)、蛋白二硫键异构酶 (PDI) 和钙连接蛋白 (CNX) 的表达水平，降低了蛋白 Hsp40 的表达水平。敲除Vd080的PDI和BiP基因后，突变体△BiP和△PDI对果胶裂解酶的敏感性降低。在没有外部压力的情况下，突变△BiP细胞中出现ER应激。果胶裂解酶影响 Vd080 细胞膜的麦角甾醇含量，从而引起 ER 应激并增加 BiP 水平以诱导 Vd080 细胞凋亡。这些结果表明果胶裂解酶可用于防治棉花黄萎病。