Abstract

Novel biomimetic zwitterionic phosphorylcholine (PC) modified bacterial cellulose (PCBC) membranes were prepared based on the Pudovik reaction of oxidized BC nanofiber clusters and then solution casting technology. Their chemical structure, morphology, hydrophilicity, mechanical and biocompatible properties were characterized and compared with non-modified BC membranes. The composition and morphology analysis of PCBC membranes confirmed the existence of zwitterionic PC groups on the membrane surfaces, and their nano-fibrous structure was kept. Compared with BC membranes, PCBC membranes exhibited better hydrophilicity, similar tensile strength, but decreased rupture strain. And PCBC membranes were non-hemolytic and non-cytotoxic like BC membranes. The anti-biofouling performance investigation revealed that PCBC membranes with zwitterionic surface showed significantly reduced protein adsorption for bovine serum albumin, as well as inhibited cell attachment for both L929 cells and macrophage RAW 264.7. The results strongly suggested that PCBC membranes with excellent cell fouling resistance are expected to be a promising anti-adhesion biomaterial.

Graphic abstract

中文翻译：

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仿生磷酸胆碱修饰的细菌纤维素膜，具有抗细胞结垢性

摘要

基于氧化的BC纳米纤维簇的Pudovik反应，然后采用溶液流延技术，制备了新型的仿生两性离子磷酸胆碱（PC）修饰的细菌纤维素（PCBC）膜。表征了它们的化学结构，形态，亲水性，机械和生物相容性，并与未改性的BC膜进行了比较。PCBC膜的组成和形态分析证实了膜表面上存在两性离子PC基，并保留了其纳米纤维结构。与BC膜相比，PCBC膜表现出更好的亲水性，相似的拉伸强度，但断裂应变降低。PCBC膜像BC膜一样具有非溶血性和无细胞毒性。抗生物污垢性能研究表明，两性离子表面的PCBC膜对牛血清白蛋白的蛋白质吸附显着降低，并且对L929细胞和巨噬细胞RAW 264.7的细胞附着均具有抑制作用。结果强烈表明，具有优异的抗细胞结垢性的PCBC膜有望成为一种有前途的抗粘附生物材料。

图形摘要