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3D Printing Hydrogel Scaffolds with Nanohydroxyapatite Gradient to Effectively Repair Osteochondral Defects in Rats
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-09-24 , DOI: 10.1002/adfm.202006697
Hui Zhang 1 , Haofei Huang 1 , Guangrun Hao 2 , Yongsheng Zhang 3 , Hao Ding 4 , Zengjie Fan 1 , Luyi Sun 4
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-09-24 , DOI: 10.1002/adfm.202006697
Hui Zhang 1 , Haofei Huang 1 , Guangrun Hao 2 , Yongsheng Zhang 3 , Hao Ding 4 , Zengjie Fan 1 , Luyi Sun 4
Affiliation
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Osteochondral (OC) defects pose an enormous challenge with no entirely satisfactory repair strategy to date. Herein, a 3D printed gradient hydrogel scaffold with a similar structure to that of OC tissue is designed, involving a pure hydrogel‐based top cartilage layer, an intermediate layer for calcified cartilage with 40% (w w−1) nanohydroxyapatite (nHA) and 60% (w w−1) hydrogel, and a 70/30% (w w−1) nHA/hydrogel‐based bottom subchondral bone layer. This study is conducted to evaluate the efficacy of the scaffold with nHA gradients in terms of its ability to promote OC defect repair. The fabricated composites are evaluated for physicochemical, mechanical, and biological properties, and then implanted into the OC defects in 56 rats. Overall, bone marrow stromal cells (BMSCs)‐loaded gradient scaffolds exhibit superior repair results as compared to other scaffolds based on gross examination, micro‐computed tomography (micro‐CT), as well as histologic and immunohistochemical analyses, confirming the ability of this novel OC graft to facilitate simultaneous regeneration of cartilage‐subchondral bone.
中文翻译：
具有纳米羟基磷灰石梯度的3D打印水凝胶支架可有效修复大鼠的骨软骨缺损。
迄今为止，骨软骨（OC）缺陷尚无完全令人满意的修复策略，这是一个巨大的挑战。本文设计了一种结构与OC组织相似的3D打印梯度水凝胶支架，包括纯水凝胶基顶部软骨层，钙化软骨的中间层，40％（ww -1）纳米羟基磷灰石（nHA）和60 ％（ww -1）水凝胶和70/30％（ww -1）基于nHA /水凝胶的软骨下软骨下层。进行这项研究以评估具有nHA梯度的支架在促进OC缺损修复方面的功效。对制成的复合材料的理化，机械和生物学特性进行评估，然后将其植入56只大鼠的OC缺损中。总体而言，基于总体检查，显微计算机断层扫描（micro-CT）以及组织学和免疫组化分析，骨髓基质细胞（BMSCs）加载的梯度支架比其他支架表现出更好的修复结果，证实了这种能力新型OC移植物，可促进软骨软骨下骨的同时再生。
更新日期：2020-09-24
中文翻译：
具有纳米羟基磷灰石梯度的3D打印水凝胶支架可有效修复大鼠的骨软骨缺损。
迄今为止，骨软骨（OC）缺陷尚无完全令人满意的修复策略，这是一个巨大的挑战。本文设计了一种结构与OC组织相似的3D打印梯度水凝胶支架，包括纯水凝胶基顶部软骨层，钙化软骨的中间层，40％（ww -1）纳米羟基磷灰石（nHA）和60 ％（ww -1）水凝胶和70/30％（ww -1）基于nHA /水凝胶的软骨下软骨下层。进行这项研究以评估具有nHA梯度的支架在促进OC缺损修复方面的功效。对制成的复合材料的理化，机械和生物学特性进行评估，然后将其植入56只大鼠的OC缺损中。总体而言，基于总体检查，显微计算机断层扫描（micro-CT）以及组织学和免疫组化分析，骨髓基质细胞（BMSCs）加载的梯度支架比其他支架表现出更好的修复结果，证实了这种能力新型OC移植物，可促进软骨软骨下骨的同时再生。
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