Background: A major focus of nanotechnology concerns is the expansion of the optimization of nanomaterials in purity, size and dispersity.

Methods: In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI water suspension and AgNP formation reaction levels.

Results: Biomass filtrate from the fungal strain Tritirachium oryzae W5H was able to reduce silver nitrate into AgNPs after a 72 h reaction, as indicated by the development of intense brown color and by UV-vis spectra. The biosynthesis ability of AgNPs was markedly better in the presence of a single carbon and nitrogen source in the culture medium compared to multiple sources of carbon and nitrogen. The optimization results of AgNP formation were indifferent between the two steps and were 20 g biomass, 40°C, pH 7.0, 96 h and 1.0 mM AgNO3. The TEM images of the prepared AgNPs illustrated the presence of 7-75 nm, monodispersed and spherical- to ovular-shaped Ag nanoparticles.

Conclusion: The present work highlights the importance of investigating the process parameters by which the reductant mycelia-free filtrate was prepared. In addition, we explored the promising antibacterial action of the prepared AgNPs against bacterial infections.

背景：纳米技术关注的焦点是扩大纳米材料在纯度，尺寸和分散性方面的优化。

方法：在目前的工作中，在菌丝体去离子水悬浮液和AgNP形成反应水平上优化了两步AgNP合成工艺。

结果：72小时反应后，米曲霉W5H菌株的生物质滤液能够将硝酸银还原为AgNPs，这是由深棕色的发展和紫外可见光谱所表明的。与多种碳和氮源相比，在培养基中存在单一碳和氮源的情况下，AgNPs的生物合成能力明显更好。AgNP形成的最优化结果在两个步骤之间无关紧要，分别为20 g生物质，40°C，pH 7.0、96 h和1.0 mM AgNO3。制备的AgNPs的TEM图像表明存在7-75 nm，单分散和球形至卵形的Ag纳米颗粒。

结论：本工作突出了研究制备无还原剂无菌丝体滤液的工艺参数的重要性。此外，我们探索了制备的AgNPs对细菌感染的有希望的抗菌作用。