Abstract In this work, 5,5′-carbonyldiisophthalic acid (H4cipa) was used as a ligand to react with Zn(NO3)2·6H2O in a mixed solvent of DMF and water to prepare a new metal-organic framework (MOF) with the chemical formula of [Zn2(cipa)(H2O)2](DMF) (1). Due to its O donor sites within the one-dimensional channels and high density of open metal sites, the activated 1a was applied as a heterogeneous catalyst for the formation of C–C bonds of carbonyl compounds in the absence of solvents, which shows high efficiency and size-selectivity. Furthermore, the possible reaction mechanism has been proposed based on the experimental results. The treatment activity of the new compound on the Mg63 osteosarcoma cell line was evaluated and the possible mechanism was discussed. First, flow cytometry was performed to detect the levels of the Mg63 cancer cells’ autophagy levels after indicated compound treatment. Next, real time RT-PCR assay was further conducted to measure the relative expression levels of the autophagic genes under treatment. Graphical abstract

中文翻译：

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酮功能化的 Zn-MOF 用于醛的无溶剂氰基硅烷化和通过增加 Mg63 细胞自噬对骨肉瘤的治疗活性

摘要 本工作以 5,5'-羰基二间苯二甲酸 (H4cipa) 为配体，在 DMF 和水的混合溶剂中与 Zn(NO3)2·6H2O 反应，制备了一种新型金属有机骨架 (MOF)。 [Zn2(cipa)(H2O)2](DMF) (1) 的化学式。由于其在一维通道内的 O 供体位点和高密度的开放金属位点，活化的 1a 被用作多相催化剂，用于在没有溶剂的情况下形成羰基化合物的 C-C 键，显示出高效率和尺寸选择性。此外，还根据实验结果提出了可能的反应机理。评估了新化合物对 Mg63 骨肉瘤细胞系的治疗活性，并讨论了可能的机制。第一的，进行流式细胞术以检测指定化合物处理后 Mg63 癌细胞的自噬水平。接下来，进一步进行实时RT-PCR测定以测量治疗下自噬基因的相对表达水平。图形概要