噬菌体对刺参海藻（Selenka）中溶藻弧菌感染的影响,Journal of the World Aquaculture Society

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噬菌体对刺参海藻（Selenka）中溶藻弧菌感染的影响
Journal of the World Aquaculture Society ( IF 2.3 ) Pub Date : 2015-04-02 , DOI: 10.1111/jwas.12177
Jiancheng Zhang _{1,

2} , Zhenhui Cao _{1,

3} , Zhen Li ₁ , Lili Wang _{1,

2} , Huaqiang Li _{2,

4} , Feifei Wu _{2,

4} , Liji Jin _{1,

2} , Xiaoyu Li ₁ , Shuying Li ₅ , Yongping Xu _{1,

2}

Affiliation

溶藻弧菌是一种重要的病原体，可在海洋动物中引起多种疾病，包括海参，刺五加（Apostichopus japonicus）。在本文中，我们描述了一种两噬菌体混合物，可能具有用作抗菌剂以预防海参中溶藻弧菌感染的潜力。从患皮肤溃疡和内脏弹出的患病海参中培养出Z1210细菌分离株。通过形态学和序列相似性分析将分离物鉴定为溶藻弧菌。随后，从水产市场的排水管中分离出两种感染分离株Z1210的噬菌体。在形态上，这两个噬菌体被分类为Podoviridae（PVA1）和在体外实验中，肌病毒科（PVA2）和每个噬菌体均显示出高毒力。此外，在海洋环境中进行的一项实验表明，两种噬菌体的混合物将海参（10±2 g）的感染复数为10、1和10时，其存活率分别提高到73％，50％和47％。或0.1。该结果与无噬菌体的对照组明显不同（存活率为3％），而两种抗生素治疗（分别为5 mg / L强力霉素和10 mg / L卡那霉素）的存活率分别为80％和47％之间没有显着差异。

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Effect of Bacteriophages on Vibrio alginolyticus Infection in the Sea Cucumber, Apostichopus japonicus (Selenka)

Vibrio alginolyticus is an important pathogen that causes a variety of diseases in marine animals including the sea cucumber, Apostichopus japonicus. Herein, we describe a two‐phage mixture which may have potential for use as an antibacterial agent to prevent V. alginolyticus infection in the sea cucumber. A Z1210 bacterial isolate was cultured from diseased sea cucumber suffering from skin ulcerations and viscera ejection. The isolate was identified as V. alginolyticus by morphology and sequence similarity analysis. Subsequently, two bacteriophages infecting isolate Z1210 were isolated from the drainpipe of an aquatic market. Morphologically, these two phages were classified as members of Podoviridae (PVA1) and Myoviridae (PVA2) and each phage showed high virulence in an in vitro experiment. Additionally, an experiment conducted in a marine environment showed that a mixture of the two phages increased the survival of sea cucumbers (10 ± 2 g) to 73, 50, and 47% when it was used with a multiplicity of infection of 10, 1, or 0.1, respectively. This result differed markedly from the control without phage (3% survival) while there was no significant difference between the 80 and 47% survival observed for two antibiotic treatments (5 mg/L doxycycline and 10 mg/L kanamycin, respectively).

更新日期：2015-04-02

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