Abstract The current research aimed to examine the effects of nano and macro iron oxide (Fe2O3) on growth performance, activity of hepatic enzymes, serum biochemical parameters, liver histopathology and appetite-related gene transcript in goldfish (Carassius auratus). Juvenile goldfish (4.3 g) were fed purified diets based on casein as a protein source and containing different levels of supplementary nano (0, 0.2, 0.5 and 1 g nFe2O3/kg diet) and macro iron oxide (0, 0.2, 0.5 and 1 g Fe2O3/kg diet) for 60 days. At the end of the experiments, blood, intestine and liver samples were obtained for histopathology, biochemical analysis and assays of ghrelin and IGF-I gene expression. The results showed that fish fed a diet supplemented with 0.5 g/kg Fe2O3 and nFe2O3 had a significantly (P .05). Histopathological analyses exhibited some liver lesions, including blood cell aggregation, Lipidosis, hyperemia in goldfish. The results revealed that administration of Fe2O3 significantly (P

中文翻译：

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纳米和宏观氧化铁 (Fe2O3) 作为饲料添加剂：对金鱼 (Carassius auratus) 生长、生化、肝酶活性、肝脏组织病理学和食欲相关基因转录的影响

摘要 目前的研究旨在研究纳米和宏观氧化铁 (Fe2O3) 对金鱼 (Carassius auratus) 生长性能、肝酶活性、血清生化参数、肝脏组织病理学和食欲相关基因转录的影响。幼年金鱼（4.3 克）饲喂以酪蛋白为蛋白质来源的纯化饲料，并含有不同水平的补充纳米（0、0.2、0.5 和 1 克 nFe2O3/kg 饲料）和大量氧化铁（0、0.2、0.5 和 1 g Fe2O3/kg 饮食）60 天。在实验结束时，获得血液、肠道和肝脏样本用于组织病理学、生化分析以及生长素释放肽和 IGF-I 基因表达的测定。结果表明，饲喂添加了 0.5 g/kg Fe2O3 和 nFe2O3 的饲料的鱼具有显着性 (P .05)。组织病理学分析显示一些肝脏病变，包括血细胞聚集、脂质沉积、金鱼充血。结果表明，Fe2O3 的施用显着（P