六种类黄酮作为新型细胞抗氧化剂的鉴定及其构效关系,Oxidative Medicine and Cellular Longevity

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六种类黄酮作为新型细胞抗氧化剂的鉴定及其构效关系
Oxidative Medicine and Cellular Longevity Pub Date : 2020-09-21 , DOI: 10.1155/2020/4150897
Qiang Zhang ₁ , Wenbo Yang ₁ , Jiechao Liu ₁ , Hui Liu ₁ , Zhenzhen Lv ₁ , Chunling Zhang ₁ , Dalei Chen ₁ , Zhonggao Jiao ₁

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这项研究旨在确定类黄酮结构与其化学和细胞内抗氧化活性的关系。通过三种不同的抗氧化剂测定法研究了60种类黄酮的抗氧化剂活性，包括2,2-二苯基-1-吡啶并肼基（DPPH）自由基清除活性，氧自由基吸收能力（ORAC）和细胞抗氧化剂活性（CAA）分析。结果显示，首次评估了6种类黄酮作为良好的细胞抗氧化剂。化合物7-甲氧基-槲皮素，3- O-甲基槲皮素，8-羟基-山emp酚，槲皮素-3- O - α-阿拉伯呋喃糖，山ka酚-7 - O-吡喃葡萄糖苷和木犀草素6- C的细胞抗氧化活性-葡萄糖苷与抗氧化酶活性（超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶）的上调有关。的结构-活性关系表明，2,3-双键，4-酮基，3 ， 4 -儿茶酚结构，并在类黄酮骨架3-羟基在抗氧化剂的行为发挥了重要作用。此外，细胞增殖测定显示对3- O-甲基槲皮素的低细胞毒性。本结果为高抗氧化剂具有不同结构的类黄酮的饮食应用提供了有价值的信息。

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Identification of Six Flavonoids as Novel Cellular Antioxidants and Their Structure-Activity Relationship

This study is aimed at determining the relationship of flavonoid structures to their chemical and intracellular antioxidant activities. The antioxidant activities of 60 flavonoids were investigated by three different antioxidant assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, oxygen radical absorption capacity (ORAC), and cellular antioxidant activity (CAA) assays. The result showed 6 flavonoids as good cellular antioxidants evaluated for the first time. The cellular antioxidant activities of compounds 7-methoxy-quercetin, 3-O-methylquercetin, 8-hydroxy-kaempferol, quercetin-3-O-α-arabinofuranose, kaempferol-7-O-glucopyranoside, and luteolin6-C-glucoside were linked with the upregulation of antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase). A structure-activity relationship suggested that 2,3-double bond, 4-keto groups, 3,4-catechol structure, and 3-hydroxyl in the flavonoid skeleton played important roles in the antioxidant behavior. Furthermore, the cell proliferative assay revealed a low cytotoxicity for 3-O-methylquercetin. The present results provide valuable information for the dietary application of flavonoids with different structures for high antioxidant.

更新日期：2020-09-21

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