To explore the function of COR gene in wheat, Wcor18 was cloned from wheat cultivar Jing 841. Expression level of Wcor18 increased with vernalization time prolongation. The full length of Wcor18 gene is 498 bp and encodes 165 amino acids. The predicted Wcor18 protein is a type of unstable hydrophilic protein and is prone to be hydrolyzed and to be oxidized. Gene structure analysis and phylogenetic tree analysis showed Wcor18 gene contains two exons and coding proteins are quite conservative among the donor ancestors of common wheat. Wcor18 can quickly respond to low temperature, high salt, drought, and ABA stresses at wheat seedling stage. The highly expression of Wcor18 gene under the − 15 °C and the rapid response of Wcor18 gene under several stress conditions indicated that the gene may play many roles in stress response of wheat.

中文翻译：

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小麦冷调控基因Wcor18的分子克隆及功能分析

为探讨COR基因在小麦中的作用，从小麦品种京841中克隆出Wcor18。Wcor18的表达水平随着春化时间的延长而增加。Wcor18基因全长498 bp，编码165个氨基酸。预测的Wcor18蛋白是一种不稳定的亲水蛋白，容易被水解和氧化。基因结构分析和系统发育树分析表明，Wcor18基因含有两个外显子，编码蛋白在普通小麦供体祖先中相当保守。Wcor18可以快速响应小麦苗期的低温、高盐、干旱和ABA胁迫。Wcor18基因在-15℃下的高表达和Wcor18基因在多种胁迫条件下的快速反应表明该基因可能在小麦的胁迫反应中发挥多种作用。