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A Simple and Regulable DNA Dimer Nanodevice to Arrange Cascade Enzymes for Sensitive Electrochemical Biosensing.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-09-17 , DOI: 10.1021/acs.analchem.0c03396 Ding Wang 1 , Yaqin Chai 1 , Yali Yuan 1 , Ruo Yuan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-09-17 , DOI: 10.1021/acs.analchem.0c03396 Ding Wang 1 , Yaqin Chai 1 , Yali Yuan 1 , Ruo Yuan 1
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Herein, a simple and regulable DNA dimer nanodevice was obtained by the assembly of two hairpin DNA monomers of H1 and H2 to control the distance between model enzymes horseradish peroxidase (HRP) and glucose oxidase (GOx) for sensitive electrochemical detection of microRNA. In detail, auto-terminated DNA polymerization reaction was designed on H1 and H2 monomers that decorated with HRP and GOx, respectively, to produce two half-released DNA monomers, which were spontaneously hybridized to each other, thereby obtaining a DNA dimer nanodevice with a rigid dsDNA linker between two DNA monomers. By varying the length of the dsDNA linker on the DNA dimer nanodevice, the distance between GOx and HRP had been regulated to the optimum and the most efficient enzyme cascade reaction was acquired for constructing a sensitive electrochemical microRNA-21 biosensor with a detection limit of 0.03 pM. In summary, the proposed DNA dimer nanodevice avoided the disadvantages of poor biocompatibility and controllability originated from traditional scleroid scaffolds and showed obvious advantages in terms of better assembly yield than previous complicated DNA scaffolds, which provided a novel strategy for developing a high-efficiency enzyme cascade catalytic system and showed great potential in other clinical diagnosis and bioanalysis application.
中文翻译：
一个简单且可调节的DNA Dimer纳米器件，用于排列级联酶以进行敏感的电化学生物传感。
本文中，通过组装H1和H2的两个发夹DNA单体来控制模型酶辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOx）之间的距离，从而实现了microRNA的灵敏电化学检测，从而获得了一种简单且可调节的DNA二聚体纳米器件。详细地，在分别用HRP和GOx装饰的H1和H2单体上设计了自动终止的DNA聚合反应，以产生两个半释放的DNA单体，它们彼此自发杂交，从而获得具有两个DNA单体之间的刚性dsDNA接头。通过改变DNA二聚体纳米器件上dsDNA接头的长度，将GOx和HRP之间的距离调节到最佳，并获得了最有效的酶级联反应，用于构建检测限为0.03 pM的敏感的电化学microRNA-21生物传感器。综上所述，拟议的DNA二聚体纳米器件避免了传统的类固醇支架产生的生物相容性和可控性差的缺点，并且在比以前复杂的DNA支架更好的组装产量方面显示出明显的优势，这为开发高效酶级联反应提供了新的策略催化系统，在其他临床诊断和生物分析应用中显示出巨大潜力。
更新日期：2020-10-21
中文翻译：
一个简单且可调节的DNA Dimer纳米器件，用于排列级联酶以进行敏感的电化学生物传感。
本文中，通过组装H1和H2的两个发夹DNA单体来控制模型酶辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOx）之间的距离，从而实现了microRNA的灵敏电化学检测，从而获得了一种简单且可调节的DNA二聚体纳米器件。详细地，在分别用HRP和GOx装饰的H1和H2单体上设计了自动终止的DNA聚合反应，以产生两个半释放的DNA单体，它们彼此自发杂交，从而获得具有两个DNA单体之间的刚性dsDNA接头。通过改变DNA二聚体纳米器件上dsDNA接头的长度，将GOx和HRP之间的距离调节到最佳，并获得了最有效的酶级联反应，用于构建检测限为0.03 pM的敏感的电化学microRNA-21生物传感器。综上所述，拟议的DNA二聚体纳米器件避免了传统的类固醇支架产生的生物相容性和可控性差的缺点，并且在比以前复杂的DNA支架更好的组装产量方面显示出明显的优势，这为开发高效酶级联反应提供了新的策略催化系统，在其他临床诊断和生物分析应用中显示出巨大潜力。
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