Single-cell RNA sequencing in Drosophila: Technologies and applications,WIREs Mechanisms of Disease

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Single-cell RNA sequencing in Drosophila: Technologies and applications
WIREs Mechanisms of Disease ( IF 4.6 ) Pub Date : 2020-09-16 , DOI: 10.1002/wdev.396
Hongjie Li ₁

Affiliation

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for investigating cell states and functions at the single-cell level. It has greatly revolutionized transcriptomic studies in many life science research fields, such as neurobiology, immunology, and developmental biology. With the fast development of both experimental platforms and bioinformatics approaches over the past decade, scRNA-seq is becoming economically feasible and experimentally practical for many biomedical laboratories. Drosophila has served as an excellent model organism for dissecting cellular and molecular mechanisms that underlie tissue development, adult cell function, disease, and aging. The recent application of scRNA-seq methods to Drosophila tissues has led to a number of exciting discoveries. In this review, I will provide a summary of recent scRNA-seq studies in Drosophila, focusing on technical approaches and biological applications. I will also discuss current challenges and future opportunities of making new discoveries using scRNA-seq in Drosophila.

中文翻译：

果蝇单细胞 RNA 测序：技术与应用

单细胞 RNA 测序 (scRNA-seq) 已成为在单细胞水平研究细胞状态和功能的强大工具。它极大地改变了神经生物学、免疫学和发育生物学等许多生命科学研究领域的转录组学研究。随着过去十年实验平台和生物信息学方法的快速发展，scRNA-seq 对于许多生物医学实验室来说变得经济可行且实验实用。果蝇是解析组织发育、成体细胞功能、疾病和衰老的细胞和分子机制的优秀模型生物。最近，scRNA-seq 方法在果蝇组织中的应用带来了许多令人兴奋的发现。在这篇综述中，我将总结最近在果蝇中的 scRNA-seq 研究，重点关注技术方法和生物学应用。我还将讨论在果蝇中使用 scRNA-seq 进行新发现的当前挑战和未来机遇。

更新日期：2020-09-16

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