Dual‐Encoded Microbeads through a Host–Guest Structure: Enormous, Flexible, and Accurate Barcodes for Multiplexed Assays,Advanced Functional Materials

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Dual‐Encoded Microbeads through a Host–Guest Structure: Enormous, Flexible, and Accurate Barcodes for Multiplexed Assays
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2016-07-18 , DOI: 10.1002/adfm.201601963
Ding Sheng-zi Zhang ₁ , Yang Jiang ₁ , Haiou Yang ₂ , Youjie Zhu ₃ , Shunjia Zhang ₁ , Ying Zhu ₁ , Dan Wei ₁ , Ye Lin ₁ , Pingping Wang ₁ , Qihua Fu ₂ , Hong Xu ₁ , Hongchen Gu ₁

Affiliation

Bead‐based suspension arrays have attracted increasing attention because of the fast growing requirements for high‐throughput multiplexed bioassays. Here, novel dual‐encoded microbeads (DEBs) based on a host–guest structure are developed. A strawberry‐like structure composed of thousands of dye‐doped nanoparticles closely packed onto the quantum dot‐encoded microbeads is designed and synthesized. Using this 3D structure, the encoded space is fully developed for multicolor encoding. As a result, a 30‐barcode library with an outstanding encoding capacity is successfully established and easily decoded via flow cytometry. The separation of organic dyes and quantum dots into two building blocks helps circumvent the optical mutual interference commonly observed in most multicolor barcodes, bringing great convenience for next‐step decoding accuracy. As a proof of concept, six typical DEBs are chosen and their feasibility in six‐plexed detection for influenza viruses is evaluated. The results show that DEBs can selectively capture the specific targets, with negligible nonspecific absorption; these results thus demonstrate the strong potential of the DEBs as a robust platform for multiplexed bioassays.

中文翻译：

通过宿主-来宾结构进行双重编码的微珠：用于多重分析的巨大，灵活而准确的条形码

基于珠的悬浮液阵列由于对高通量多重生物测定法的快速增长的需求而引起了越来越多的关注。在这里，开发了一种基于宿主-客体结构的新型双编码微珠（DEB）。设计并合成了由数千个染料掺杂的纳米颗粒紧密堆积在量子点编码的微珠上的草莓状结构。使用此3D结构，可以充分开发编码空间以进行多色编码。结果，成功建立了具有出色编码能力的30条形码库，并可以通过流式细胞仪轻松解码。将有机染料和量子点分为两个构件有助于避免大多数多色条形码中常见的光学相互干扰，为下一步解码的准确性带来极大的便利。作为概念验证，选择了六个典型的DEB，并评估了它们在六重检测流感病毒中的可行性。结果表明，DEBs可以选择性地捕获特定目标，而非特异性吸收可忽略不计。因此，这些结果证明了DEB作为多元生物测定的强大平台的强大潜力。

更新日期：2016-07-18

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