Ultraviolet radiation B cause photoaging of the skin by enhancing the high expression of matrix metalloproteinases (MMPs) to degrade collagen and inhibit the synthesis of procollagen. Trehalose (TRE), a widely occurring natural disaccharide, has been shown to play a cytoprotective role in many pathological processes. However, few studies have evaluated whether it has anti-photoaging potential. In this study, we investigated the anti-photoaging and mechanism of action of TRE using Human immortalized keratinocytes (HaCaT) cells. The results indicated that TRE pretreatment effectively scavenges reactive oxygen species (ROS) accumulation induced by UVB and increases the content of certain endogenous antioxidant factors in HaCaT cells. TRE suppressed UVB-induced MMP expression by blocking mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1) and NF-κB signaling. TRE treatment increased procollagen I synthesis via activating transforming growth factor-β (TGF-β)/Smad pathway and demonstrated that TRE may be a potential natural disaccharide for photoaging therapy in the future.

紫外线B通过增强基质金属蛋白酶（MMP）的高表达来降解胶原蛋白并抑制胶原蛋白的合成，从而引起皮肤光老化。海藻糖（TRE）是一种广泛存在的天然二糖，已显示在许多病理过程中起细胞保护作用。但是，很少有研究评估它是否具有抗光老化的潜力。在这项研究中，我们调查了人类永生化角质形成细胞（HaCaT）细胞对TRE的抗光老化作用和作用机理。结果表明，TRE预处理可有效清除UVB诱导的活性氧（ROS）积累，并增加HaCaT细胞中某些内源性抗氧化剂的含量。TRE通过阻断有丝分裂原激活的蛋白激酶（MAPK）抑制UVB诱导的MMP表达，激活蛋白1（AP-1）和NF-κB信号传导。TRE治疗通过激活转化生长因子-β（TGF-β）/ Smad途径增加了前胶原I的合成，并证明TRE可能是将来用于光老化疗法的潜在天然二糖。