Abstract Currently essential oils are increasingly used as natural insecticides because of the adverse effects on human health and environmental pollution of synthetic insecticides. The aim of this study is to perform the encapsulation of Ocimum basilicum L. essential oils by freeze-drying technique using binary mixtures maltodextrins and acacia gum to investigate their insecticidal activity against three insect pests of stored-products Rhyzopertha dominica, Sitophilus oryzae and Tribolium castaneum by direct contact and ingestion toxicity effects. Essential oils were obtained by solvent-free microwave extraction (SFME) and conventional hydro-distillation (HD) methods. Chemical composition of essential oils analysed by GC-MS indicated that the major components were linalool (43.5% for SFME and 48.4% for HD) followed by methyl chavicol (13.3% for SFME and 14.3% for HD) and 1,8-cineole (6.8% for SFME and 7.3% for HD). The toxic effects results, confirm that O. basilicum essential oils capsules were highly effective against R. dominica than S. oryzae, except for T. castaneum which was insensitive. The toxicity against adults of R. dominica varied between (LD50 = 0.20 g/kg for SFME-EEO, LD50 = 0.25 g/kg for HD-EEO) and (LD50 = 0.32 g/kg for SFME-EEO, LD50 = 0.37 g/kg for HD-EEO) by direct contact and ingestion effect, respectively. These results lead us to consider such essential oils capsules as providing a richer source of natural insecticide agents for food preservation.

中文翻译：

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无溶剂微波提取，然后封装 O. basilicum L. 精油用于杀虫目的

摘要 目前，由于合成杀虫剂对人体健康和环境污染等不利影响，精油作为天然杀虫剂被越来越多地使用。本研究的目的是使用麦芽糊精和阿拉伯胶的二元混合物通过冷冻干燥技术对罗勒精油进行封装，以研究它们对三种贮藏产品Rhyzopertha dominica、Sitophilus oryzae和Tribolium castaneum的杀虫活性。通过直接接触和摄入毒性作用。精油是通过无溶剂微波萃取 (SFME) 和常规水力蒸馏 (HD) 方法获得的。GC-MS 分析的精油化学成分表明，主要成分是芳樟醇（SFME 为 43.5%，HD 为 48.4%），其次是甲基鱼尾醇（13. SFME 为 3%，HD 为 14.3%）和 1,8-桉树脑（SFME 为 6.8%，HD 为 7.3%）。毒性作用结果证实，除了不敏感的 T. castaneum 之外，O. basilicum 精油胶囊对 R. dominica 的效果高于 S. oryzae。对 R. dominica 成虫的毒性在（SFME-EEO 的 LD50 = 0.20 g/kg，HD-EEO 的 LD50 = 0.25 g/kg）和（SFME-EEO 的 LD50 = 0.32 g/kg，LD50 = 0.37 g /kg for HD-EEO) 分别通过直接接触和摄入效应。这些结果使我们认为这种精油胶囊为食品保存提供了更丰富的天然杀虫剂来源。除了不敏感的T. castaneum。对 R. dominica 成虫的毒性在（SFME-EEO 的 LD50 = 0.20 g/kg，HD-EEO 的 LD50 = 0.25 g/kg）和（SFME-EEO 的 LD50 = 0.32 g/kg，LD50 = 0.37 g /kg for HD-EEO) 分别通过直接接触和摄入效应。这些结果使我们认为这种精油胶囊为食品保存提供了更丰富的天然杀虫剂来源。除了不敏感的T. castaneum。对 R. dominica 成虫的毒性在（SFME-EEO 的 LD50 = 0.20 g/kg，HD-EEO 的 LD50 = 0.25 g/kg）和（SFME-EEO 的 LD50 = 0.32 g/kg，LD50 = 0.37 g /kg for HD-EEO) 分别通过直接接触和摄入效应。这些结果使我们认为这种精油胶囊为食品保存提供了更丰富的天然杀虫剂来源。