Ergocalciferol (VD2) and cholecalciferol (VD3) are bioactive compounds with reduced bioavailability. Soybean oil, olive oil, or medium chain triglyceride containing 0.5% w/w VD2 and VD3, was used as a dispersed phase, while phosphate buffer containing 1% w/w Tween 20 served as a continuous phase. The two phases were emulsified with a rotor-stator homogenizer (RSH) at 5000–20 000 rpm for 5 min or with high pressure homogenization (HPH) at 100 MPa after RSH at 7000 rpm for 5 min. The volume mean diameter (d4,3) of the formulated oil-in-water (O/W) emulsions gradually decreased with increasing the rotation speed of RSH. The O/W emulsions formulated under appropriate homogenization conditions remained stable over 30 days of storage period. There was no prominent difference in d4,3 of the O/W emulsions formulated with the HPH, differing from d4,3 of the O/W emulsions formulated with the RSH during 30 days of storage period. There was little effect of homogenization types on release profile of the VD2 and VD3 loaded emulsions. The encapsulation efficiencies of VD2 and VD3 were less than 70% after 10 days of storage, followed by sharp decline to 10% after 30 days at 4°C. Practical applications: Multiple studies have emphasized the benefits to human health of including vitamins in the diet and as special nutrient formula for athletes. For this reason, our study was proposed to formulate a stable emulsion system that could later be added to formulate high energy food or energy drinks. A mixture of cholecalciferol (VD2) and ergocalciferol (VD3) is successfully encapsulated in the O/W emulsions. The different homogenization conditions have no significant effect on the encapsulation efficiencies. There is sharp decline in encapsulation efficiencies of VD2 and VD3 after 15 days of storage.

中文翻译：

---

通过不同的均质化技术将胆钙化醇和麦角钙化醇封装在水包油乳剂中

麦角钙化醇 (VD2) 和胆钙化醇 (VD3) 是具有降低生物利用度的生物活性化合物。大豆油、橄榄油或含有 0.5% w/w VD2 和 VD3 的中链甘油三酯用作分散相，而含有 1% w/w Tween 20 的磷酸盐缓冲液用作连续相。两相用转子 - 定子均质机（RSH）在 5000-20 000 rpm 下乳化 5 分钟，或在 7000 rpm 下 RSH 5 分钟后，在 100 MPa 下用高压均质器（HPH）乳化。随着RSH转速的增加，配制的水包油(O/W)乳液的体积平均直径(d4,3)逐渐减小。在适当的均质条件下配制的 O/W 乳液在 30 天的储存期内保持稳定。用 HPH 配制的 O/W 乳剂的 d4,3 没有显着差异，与 d4、在 30 天的储存期内，使用 RSH 配制的 3 种 O/W 乳液。均质类型对 VD2 和 VD3 负载乳液的释放曲线几乎没有影响。VD2 和 VD3 的包封率在储存 10 天后低于 70%，随后在 4°C 下储存 30 天后急剧下降至 10%。实际应用：多项研究强调了在饮食中加入维生素以及作为运动员的特殊营养配方对人类健康的益处。出于这个原因，我们的研究被提议配制一种稳定的乳液系统，以后可以将其添加到配制高能量食品或能量饮料中。胆钙化醇 (VD2) 和麦角钙化醇 (VD3) 的混合物成功地封装在 O/W 乳液中。不同的均质化条件对封装效率没有显着影响。储存 15 天后，VD2 和 VD3 的封装效率急剧下降。