Herein, an amplified electrochemiluminescence (ECL) ratiometric biosensor for p53 DNA sequence assay was described based on gold@carbon dots nanoflowers (Au@CDs), gold@luminol nanoparticles (Au@luminol) as luminophors and an enzyme-free DNA walker for signal amplification. The ratiometric strategy endowed the sensor with a prominent accuracy. Taking advantage of DNA-walker signal amplification feature, a high sensitivity for target detection was achieved. Under the optimal conditions, the designed biosensor exhibited a lower limit of 0.34 fM and wide linear range from 1 fM to 10 pM for p53 DNA sequence detection. In particular, compared with traditional Ru(bpy)₃³⁺/Ru(bpy)₃²⁺ derivatives and semiconductor nanoparticles, Au@luminol and Au@CDs have the advantages of cost-effective, excellent biocompatibility, and easy labeling, which offered new opportunity for building ratiometric ECL biosensor platform.

本文中，基于金@碳点纳米花（Au @ CDs），金@鲁米诺纳米颗粒（Au @ luminol）作为发光体和无酶DNA Walker信号，描述了一种用于p53 DNA序列测定的放大电化学发光（ECL）比例生物传感器。放大。比例策略使传感器具有卓越的精度。利用DNA-walker信号放大功能，实现了目标检测的高灵敏度。在最佳条件下，所设计的生物传感器的下限为0.34 fM，线性范围为1 fM至10 pM，可用于p53 DNA序列检测。特别是与传统的Ru（bpy）₃ ³⁺ / Ru（bpy）₃ ²⁺ 衍生物和半导体纳米粒子，Au @ luminol和Au @ CDs具有成本效益高，生物相容性强，易于标记的优点，这为构建比例ECL生物传感器平台提供了新的机会。