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The Milestone of Genetic Screening: Mammalian Haploid Cells
Computational and Structural Biotechnology Journal ( IF 4.4 ) Pub Date : 2020-09-12 , DOI: 10.1016/j.csbj.2020.09.006
Shengyi Sun 1 , Yiding Zhao 1 , Ling Shuai 1, 2, 3
Affiliation  

Mammalian haploid cells provide insights into multiple genetics approaches as have been proved by advances in homozygous phenotypes and function as gametes. Recent achievements make ploidy of mammalian haploid cells stable and improve the developmental efficiency of embryos derived from mammalian haploid cells intracytoplasmic microinjection, which promise great potentials for using mammalian haploid cells in forward and reverse genetic screening. In this review, we introduce breakthroughs of mammalian haploid cells involving in mechanisms of self-diploidization, forward genetics for various targeting genes and imprinted genes related development.



中文翻译:


基因筛查的里程碑:哺乳动物单倍体细胞



哺乳动物单倍体细胞为多种遗传学方法提供了见解,纯合表型和配子功能的进步已经证明了这一点。近年来的研究成果使哺乳动物单倍体细胞的倍性稳定,并提高了哺乳动物单倍体细胞胞浆内显微注射衍生胚胎的发育效率,这为利用哺乳动物单倍体细胞进行正向和反向遗传筛选提供了巨大的潜力。在这篇综述中,我们介绍了哺乳动物单倍体细胞在自二倍化机制、各种靶向基因的正向遗传学和印记基因相关发育方面的突破。

更新日期:2020-09-12
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