Synthesis of novel 4(3H)-quinazolinonyl aminopyrimidine derivatives has been achieved via quinazolinonyl enones which in turn were obtained from 2-acyl-4(3H)-quinazolinone. They have been assayed for biofilm inhibition against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative bacteria (Acinetobacter baumannii). The analogues with 2,4,6-trimethoxy phenyl, 4-methylthio phenyl, and 3-bromo phenyl substituents (5h, 5j & 5k) have been shown to inhibit biofilm formation efficiently in MRSA with IC₅₀ values of 20.7–22.4 μM). The analogues 5h and 5j have demonstrated low toxicity in human cells in vitro and can be investigated further as leads.

通过喹唑啉酮烯酮合成了新型 4(3 H )-喹唑啉酮基氨基嘧啶衍生物，而喹唑啉酮烯酮又从 2-酰基-4(3 H )-喹唑啉酮获得。已对它们进行了针对革兰氏阳性菌（耐甲氧西林金黄色葡萄球菌(MRSA)）和革兰氏阴性菌（鲍曼不动杆菌）的生物膜抑制试验。具有 2,4,6-三甲氧基苯基、4-甲硫基苯基和 3-溴苯基取代基的类似物（5h、5j和5k）已被证明可以有效抑制 MRSA 中的生物膜形成，IC ₅₀值为 20.7–22.4 μM） 。类似物5h和5j在体外人体细胞中显示出低毒性，可以作为先导化合物进行进一步研究。