The study investigated the antifungal activity and potential antifungal mechanisms of volatile compounds (i.e., E‐2‐hexenal) against Penicillium cyclopium, one of the main tomato postharvest pathogens, which was obtained and purified from postharvest tomato surface. Experimental data suggested that the volatile compound exhibited strong antifungal activity against the targeted pathogens, with minimum inhibitory concentration and minimum fungicidal concentration of 160 μL/L and 320μL/L for E‐2‐hexenal, respectively. The membrane permeability of the P. cyclopium increased with increasing concentrations of E‐2‐hexenal, as evidenced by cell constituent release, leakage of potassium ions, and extracellular conductivity. Moreover, E‐2‐hexenal could induce a decrease in total lipid content and extracellular pH. These results suggest that the anti‐fungal activity of E‐2‐hexenal against P. cyclopium can be attributed to the disruption of the cell membrane integrity, the increase of membrane permeability and the leakage of cell components.

中文翻译：

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反式-2-己烯醛对青霉环菌的膜活性机理研究

该研究调查了挥发性化合物（即E-2-己烯醛）对青贮藏后病原体之一的青霉环菌的抑菌活性和潜在的抑菌机理，青贮菌是从贮藏后的番茄表面获得并纯化的。实验数据表明，该挥发性化合物对目标病原体具有较强的抗真菌活性，对E-2-己烯二酚的最低抑菌浓度和最小杀真菌浓度分别为160μL/ L和320μL/ L。环孢假单胞菌的膜通透性随着E-2-己烯醛浓度的增加而增加，这表现为细胞成分释放，钾离子泄漏和细胞外电导率。此外，E-2-己烯醛可能导致总脂质含量和细胞外pH降低。这些结果表明，E-2-己烯醛对环孢假单胞菌的抗真菌活性可归因于细胞膜完整性的破坏，膜通透性的增加和细胞成分的泄漏。