Self-Assembled Peptide Nanofibrils Designed to Release Membrane-Lysing Antimicrobial Peptides,ACS Applied Bio Materials

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Self-Assembled Peptide Nanofibrils Designed to Release Membrane-Lysing Antimicrobial Peptides
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-05-15 00:00:00 , DOI: 10.1021/acsabm.0c00281
Xiangyu Sha ₁ , Ping Li ₂ , Yonghai Feng ₁ , Dan Xia ₃ , Xiaohua Tian ₁ , Zengkai Wang ₁ , Yanlian Yang ₂ , Xiaobo Mao _{4,

5} , Lei Liu ₁

Affiliation

Membrane-disrupting antimicrobial peptides continue to attract increasing attention due to their potential to combat multidrug-resistant bacteria. However, some limitations are found in the success of clinical setting-based antimicrobial peptide agents, for instance, the poor stability of antimicrobial peptides in vivo and their short-term activity. Self-assembled peptide materials can improve the stability of antimicrobial peptides, but the biosafety of peptide-based materials is the main concern, although they are considered to be biocompatible, because some peptide aggregates would possibly induce protein misfolding, which could be related to amyloid-related diseases. Therefore, in this work, we designed two peptides and constructed peptide-based nanofibrils by self-assembly before its utilization. It is found that the fibrils could release the antimicrobial peptide by disassembly for microbial membrane lysis in the presence of bacteria. The designed peptide-based fibrils presented a good and long-term antimicrobial activity with bacterial membrane disruption and the efflux of calcium from bacteria. Furthermore, it could be used to construct hybrid macrofilms displaying low cytotoxicity, low hemolytic activity, and good biocompatibility. The innovative design strategy could be beneficial for the development of smart antimicrobial nanomaterials.

中文翻译：

自组装肽纳米纤丝设计用于释放膜裂解的抗菌肽

破坏膜的抗微生物肽由于其对抗多药耐药细菌的潜力而继续受到越来越多的关注。然而，在基于临床环境的抗微生物肽试剂的成功中发现了一些局限性，例如，抗微生物肽在体内的较差的稳定性及其短期活性。自组装肽材料可以提高抗菌肽的稳定性，但是基于肽的材料的生物安全性是主要关注的问题，尽管它们被认为具有生物相容性，因为某些肽聚集体可能会引起蛋白质错误折叠，这可能与淀粉样蛋白有关。相关疾病。因此，在这项工作中，我们设计了两种肽并在利用前通过自组装构建了基于肽的纳米原纤维。发现在细菌的存在下，原纤维可以通过分解用于微生物膜裂解而释放抗微生物肽。设计的基于肽的原纤维具有良好的长期抗菌活性，具有细菌膜破裂和钙从细菌外排的作用。此外，它可用于构建显示低细胞毒性，低溶血活性和良好生物相容性的杂合大薄膜。创新的设计策略可能有益于智能抗菌纳米材料的开发。它可用于构建具有低细胞毒性，低溶血活性和良好生物相容性的杂交大分子膜。创新的设计策略可能有益于智能抗菌纳米材料的开发。它可用于构建具有低细胞毒性，低溶血活性和良好生物相容性的杂交大分子膜。创新的设计策略可能有益于智能抗菌纳米材料的开发。

更新日期：2020-05-15

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