基于发光MOF的生物传感器对α-酮戊二酸疾病标记物的多值逻辑测定,ACS Applied Bio Materials

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基于发光MOF的生物传感器对α-酮戊二酸疾病标记物的多值逻辑测定
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-05-18 00:00:00 , DOI: 10.1021/acsabm.0c00378
Xu Dai ₁ , Ji-Na Hao ₁ , Jinlou Gu ₁ , Yongsheng Li ₁

Affiliation

α-酮戊二酸（α-KA）是克雷布斯循环中重要的内源性代谢产物，被认为是各种疾病的重要标志。已经报道了几种鉴定α-KA的方法。但是，它们中的大多数只限于一种测定中的单信号转导模式，并且在悬浮状态下工作，这很容易引起定量误差并导致假阳性检测结果。本文首先设计和制造了一种多值响应半固体EuMOF-gel（EuMOG）逻辑生物传感器，它可以实现与疾病相关的α标记物的特异性，快速和易于区分的裸眼检测。通过使用三个多色门执行一对一逻辑运算，实现血清中的-KA。可以通过组装2来合理地实现一对一逻辑运算的实现，³⁺离子作为红色发射输出2，α-KA刺激物作为输入和能量阻滞剂，在双光谱分解输出合并为一个单元之间。有了各种分析物之间特定的α-KA输入，逻辑解码器的二进制代码就会从（0,1）切换到（1,0），并且颜色会从红色变为青色。此外，通过将输出色度集成到逻辑运算中，可以使用智能EuMOG检测器轻松评估α-KA的浓度水平，该检测器具有并行编码低（0,1），正常（1,1）的三个多色门以及危险（1,0）状态。这种多值逻辑生物传感器提供了一种简便的策略来提高疾病相关生物标记物的分析可靠性。

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Multivalued Logic Assay of the Disease Marker of α-Ketoglutaric Acid by a Luminescent MOF-Based Biosensor

α-Ketoglutaric acid (α-KA) is an important endogenous metabolite in the Krebs cycle and considered a critical marker for various diseases. Several approaches for identifying α-KA have been reported. However, most of them are limited to single-signal transduction modes in one assay and working in the suspension state, which easily cause quantification errors and lead to false-positive detection results. Herein, a multivalue-responsive and semi-solid EuMOF-gel (EuMOG) logic biosensor is first designed and fabricated, which can realize the specific, rapid, and easy-to-differentiate naked-eye detection of the disease-associated marker of α-KA in serum through implementing one-to-two logic operation with three multicolor gates. The implementation of one-to-two logic operation can be rationally achieved by assembling 2,6-naphthalenedicarboxylic acid as both the energy donor and blue-colored output 1, photoactive Eu³⁺ ions as the red-emissive output 2, and α-KA stimuli as an input and energy blocker between the dual spectrum-resolved outputs into one unit. With the specific input of α-KA among various analytes, the binary code of the logic decoder switches from (0,1) to (1,0) with an obvious color change from red to cyan. Moreover, by integrating the output chromaticity into the logical operations, the concentration levels of α-KA could be easily evaluated with the intelligent EuMOG detector, which has three multicolor gates in parallel encoding low (0,1), normal (1,1), and danger (1,0) statuses. Such multivalued logic biosensor provides a facile strategy to improve the analysis reliability of disease-associated biomarkers.

更新日期：2020-05-18

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