时间分辨拉曼光谱抑制空间相关样品背景荧光的评价,Journal of Raman Spectroscopy

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时间分辨拉曼光谱抑制空间相关样品背景荧光的评价
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2019-05-21 , DOI: 10.1002/jrs.5586
Franziska Hanke ₁ , Bram J.A. Mooij ₂ , Freek Ariese ₂ , Ute Böttger ₁

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太空研究的主要目标之一是寻找现存或灭绝的外星生命迹象，拉曼光谱可以在这一领域发挥作用。拉曼光谱仪计划用于未来的火星和月球任务，以识别矿物表面成分和潜在的有机化合物（尤其是在火星上）。然而，拉曼光谱的一个主要挑战，尤其是在可见光范围内，是强烈的荧光背景。时间分辨拉曼光谱 (TRRS) 可以提供对拉曼信号的选择性检测，而不是通常寿命更长的荧光。本研究调查了 TRRS 系统的潜力，使用 3 ps、440 nm 激光脉冲和带有增强电荷耦合器件 (CCD) 相机的时间门控检测。测试样品是作为外星生命模拟物的线虫地衣，以及作为行星表面模拟物的月球风化层模拟材料 (LRS)。TRRS 技术是通过比较门控与非门控拉曼光谱使用不同的检测器，但在其他方面使用相同的仪器和相同的测量条件来评估的。与非门控光谱相比，线虫的门控光谱显示出显着的信噪比 (SNR) 改进。可见的拉曼线可以归于光保护色素帕列汀。对于 LRS 样品，在门控模式下测量并没有显着改善非门控光谱中具有良好 SNR 的测量点。然而，由于荧光抑制，以荧光为主的斑点在门控模式中显示出显着改善。矿物，如斜长石、透辉石、检测到橄榄石、磷灰石和碳酸盐矿物。在大多数情况下，与非门控测量相比，TRRS 提供了更好的结果，证明了未来太空探索任务的适用性。

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The evaluation of time‐resolved Raman spectroscopy for the suppression of background fluorescence from space‐relevant samples

One of the primary goals in space research is the search for signs of extant or extinct extraterrestrial life, and Raman spectroscopy can play a role in this field. Raman spectrometers are planned for future missions to Mars and possibly the Moon to identify the mineralogical surface composition and potentially existing organic compounds (especially on Mars). However, a major challenge in Raman spectroscopy, especially in the visible range, is the strong fluorescence background. Time‐resolved Raman spectroscopy (TRRS) can provide selective detection of Raman signals over the generally longer living fluorescence. This study investigates the potential of a TRRS system, using 3‐ps, 440‐nm laser pulses and time‐gated detection with an intensified charge‐coupled device (CCD) camera. Test samples were the lichen Xanthoria elegans as an extraterrestrial life analogue, and a lunar regolith analogue material (LRS) as a planetary surface analogue. The TRRS technique is evaluated by comparing gated to nongated Raman spectroscopy using different detectors but with otherwise the same instrument and identical measurement conditions. The gated spectra of X. elegans showed significant signal‐to‐noise ratio (SNR) improvements compared to the nongated spectra. The visible Raman lines could be assigned to the photoprotective pigment parietin. For the LRS sample, measurement spots with a good SNR in the nongated spectrum were not significantly improved by measuring in gated mode. However, spots dominated by fluorescence showed significant improvement in gated mode because of fluorescence suppression. Minerals such as plagioclase, diopside, olivine, apatite, and a carbonate mineral were detected. In most cases, TRRS provided better results compared to nongated measurements, demonstrating the suitability for future space‐exploration missions.

更新日期：2019-05-21

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