Epimagnolin A 通过抑制 PMA 刺激的 THP-1 细胞中的 p38/NF-κB 和 AP-1 信号通路来抑制 IL-6 的产生,Environmental Toxicology

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Epimagnolin A 通过抑制 PMA 刺激的 THP-1 细胞中的 p38/NF-κB 和 AP-1 信号通路来抑制 IL-6 的产生
Environmental Toxicology ( IF 4.4 ) Pub Date : 2019-03-27 , DOI: 10.1002/tox.22746
Hyun‐Woo Chun ₁ , Soo‐Jin Kim ₁ , Thu‐Huyen Pham ₁ , Yesol Bak ₁ , Jaewook Oh ₂ , Hyung‐Won Ryu ₃ , Sei‐Ryang Oh ₃ , Jin‐Tae Hong ₄ , Do‐Young Yoon ₁

Affiliation

Epimagnolin A 是从 Magnolia fargesii 的花蕾中提取的木脂素，传统上用于亚洲医学治疗头痛和鼻塞。在先前的研究中，从 M. fargesii 中提取的一种草药化合物 Fargesin 对人类单核细胞 THP-1 细胞具有抗炎作用。然而，表木兰素 A 的抗炎作用尚未阐明。在这项研究中，证明表木素 A 降低了佛波醇-12-肉豆蔻酸酯-13-乙酸 (PMA) 诱导的 IL-6 启动子活性和人单核细胞 THP-1 细胞中 IL-6 的产生。此外，还研究了表木兰素 A 对丝裂原活化蛋白激酶、核因子-κB (NF-κB) 和激活蛋白 1 (AP-1) 活性的调节作用。p38 的磷酸化以及 p50 和 c-Jun 的核转位在 PMA 刺激的 THP-1 细胞中被表木酚 A 下调。结果表明，表木兰素 A 通过 PMA 刺激的 THP-1 中的 p38/NF-kB 和 AP-1 信号通路减弱了 NF-κB 和 AP-1 转录因子对 IL-6 启动子和 IL-6 产生的结合亲和力。细胞。这些结果表明表木兰素 A 可以成为治疗炎症性疾病的有用药物。

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Epimagnolin A inhibits IL‐6 production by inhibiting p38/NF‐κB and AP‐1 signaling pathways in PMA‐stimulated THP‐1 cells

Epimagnolin A is a lignan obtained from the flower buds of Magnolia fargesii, which is traditionally used in Asian medicine for treating headache and nasal congestion. A herbal compound fargesin obtained from M. fargesii, has exerted anti‐inflammatory effects in human monocytic THP‐1 cells in the previous study. The anti‐inflammatory effects of epimagnolin A, however, have been not elucidated yet. In this study, it was demonstrated that epimagnolin A reduced phorbol‐12‐myristate‐13‐acetate (PMA)‐induced IL‐6 promoter activity and IL‐6 production in human monocytic THP‐1 cells. Furthermore, it was investigated the modulating effects of epimagnolin A on mitogen‐activated protein kinase, nuclear factor‐kappa B (NF‐κB), and activator protein 1 (AP‐1) activities. Phosphorylation of p38 and nuclear translocation of p50 and c‐Jun were down‐regulated by epimagnolin A in the PMA‐stimulated THP‐1 cell. The results revealed that epimagnolin A attenuated the binding affinity of NF‐κB and AP‐1 transcription factors to IL‐6 promoter and IL‐6 production through p38/NF‐kB and AP‐1 signaling pathways in the PMA‐stimulated THP‐1 cells. These results suggest that epimagnolin A can be a useful drug for the treatment of inflammatory diseases.

更新日期：2019-03-27

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