当前位置：
X-MOL 学术
›
Microchim. Acta
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Paper chip-based colorimetric assay for detection of Salmonella typhimurium by combining aptamer-modified Fe3O4@Ag nanoprobes and urease activity inhibition
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-09 , DOI: 10.1007/s00604-020-04537-8 Shengnan Wei 1 , Juan Li 1 , Jingya He 2 , Wei Zhao 3 , Feng Wang 2 , Xiuling Song 1, 4 , Kun Xu 1, 4 , Juan Wang 1 , Chao Zhao 1
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-09 , DOI: 10.1007/s00604-020-04537-8 Shengnan Wei 1 , Juan Li 1 , Jingya He 2 , Wei Zhao 3 , Feng Wang 2 , Xiuling Song 1, 4 , Kun Xu 1, 4 , Juan Wang 1 , Chao Zhao 1
Affiliation
|
A rapid and sensitive colorimetric assay is described for Salmonella typhimurium (S. typhimurium) detection using urea/phenol red impregnated test paper. Aptamer-modified Fe3O4@Ag multifunctional hybrid nanoprobes (apt-Fe3O4@Ag NPs) were used to specifically captured S. typhimurium; the nanoprobes were quickly etched by H2O2 to form Ag+. The generated Ag+ can inhibit the urease-catalyzed hydrolysis reaction of urea to produce NH4+. Consequently, the as-prepared test paper displayed a yellow color. In the presence of S. typhimurium, the target bacteria can cause aggregation of apt-Fe3O4@Ag NPs, and the deposited Ag on the nanoprobe’s surface is shielded against H2O2-induced oxidative decomposition leading to reduced Ag+ production. The catalytic activity of urease cannot be inhibited completely by inadequate amount of Ag+. An obvious color change from yellow to pink can be monitored directly using our test paper as a result of increased NH4+. The entire assay procedure could be completed within 1 h. A limit of detection of 48 cfu/mL is achieved with a linear range of 1 × 102 to 1 × 106 cfu/mL. The recoveries of S. typhimurium spiked in pure milk samples were 92.48–94.05%. Graphical abstract Schematic diagram of the proposed colorimetric assay for S. typhimurium detection based on etching of bifunctional apt-Fe3O4@Ag NPs and inhibiting catalytic activity of urease by Ag+. A color change from yellow to pink can be observed and correlated to the concentration of S. typhimurium. Schematic diagram of the proposed colorimetric assay for S. typhimurium detection based on etching of bifunctional apt-Fe3O4@Ag NPs and inhibiting catalytic activity of urease by Ag+. A color change from yellow to pink can be observed and correlated to the concentration of S. typhimurium.
中文翻译：
基于纸芯片的比色法检测结合适体修饰的 Fe3O4@Ag 纳米探针和脲酶活性抑制鼠伤寒沙门氏菌
描述了使用尿素/酚红浸渍试纸检测鼠伤寒沙门氏菌 (S. typhimurium) 的一种快速灵敏的比色法。适体修饰的 Fe3O4@Ag 多功能杂化纳米探针（apt-Fe3O4@Ag NPs）用于特异性捕获鼠伤寒沙门氏菌；纳米探针被 H2O2 快速蚀刻以形成 Ag+。生成的Ag+可以抑制尿素酶催化的尿素水解反应生成NH4+。因此，所制备的试纸显示黄色。在鼠伤寒沙门氏菌的存在下，目标细菌会导致 apt-Fe3O4@Ag NPs 的聚集，并且纳米探针表面上沉积的 Ag 被屏蔽以防止 H2O2 诱导的氧化分解，从而导致 Ag+ 的产生减少。尿素酶的催化活性不能被不足的Ag+量完全抑制。由于 NH4+ 增加，可以使用我们的试纸直接监测从黄色到粉红色的明显颜色变化。整个检测过程可在 1 小时内完成。检测限为 48 cfu/mL，线性范围为 1 × 102 至 1 × 106 cfu/mL。纯牛奶样品中加标鼠伤寒沙门氏菌的回收率为 92.48–94.05%。基于蚀刻双功能 apt-Fe3O4@Ag NPs 和抑制 Ag+ 对脲酶的催化活性，拟议的鼠伤寒沙门氏菌比色检测示意图。可以观察到从黄色到粉红色的颜色变化，并与鼠伤寒沙门氏菌的浓度相关。基于双功能 apt-Fe3O4@Ag NPs 的蚀刻和 Ag+ 抑制脲酶催化活性的拟议的鼠伤寒沙门氏菌检测比色法的示意图。
更新日期：2020-09-09
中文翻译：
基于纸芯片的比色法检测结合适体修饰的 Fe3O4@Ag 纳米探针和脲酶活性抑制鼠伤寒沙门氏菌
描述了使用尿素/酚红浸渍试纸检测鼠伤寒沙门氏菌 (S. typhimurium) 的一种快速灵敏的比色法。适体修饰的 Fe3O4@Ag 多功能杂化纳米探针（apt-Fe3O4@Ag NPs）用于特异性捕获鼠伤寒沙门氏菌；纳米探针被 H2O2 快速蚀刻以形成 Ag+。生成的Ag+可以抑制尿素酶催化的尿素水解反应生成NH4+。因此，所制备的试纸显示黄色。在鼠伤寒沙门氏菌的存在下，目标细菌会导致 apt-Fe3O4@Ag NPs 的聚集，并且纳米探针表面上沉积的 Ag 被屏蔽以防止 H2O2 诱导的氧化分解，从而导致 Ag+ 的产生减少。尿素酶的催化活性不能被不足的Ag+量完全抑制。由于 NH4+ 增加，可以使用我们的试纸直接监测从黄色到粉红色的明显颜色变化。整个检测过程可在 1 小时内完成。检测限为 48 cfu/mL，线性范围为 1 × 102 至 1 × 106 cfu/mL。纯牛奶样品中加标鼠伤寒沙门氏菌的回收率为 92.48–94.05%。基于蚀刻双功能 apt-Fe3O4@Ag NPs 和抑制 Ag+ 对脲酶的催化活性，拟议的鼠伤寒沙门氏菌比色检测示意图。可以观察到从黄色到粉红色的颜色变化，并与鼠伤寒沙门氏菌的浓度相关。基于双功能 apt-Fe3O4@Ag NPs 的蚀刻和 Ag+ 抑制脲酶催化活性的拟议的鼠伤寒沙门氏菌检测比色法的示意图。
京公网安备 11010802027423号