m6A transferase METTL3‐induced lncRNA ABHD11‐AS1 promotes the Warburg effect of non‐small‐cell lung cancer,Journal of Cellular Physiology

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m6A transferase METTL3‐induced lncRNA ABHD11‐AS1 promotes the Warburg effect of non‐small‐cell lung cancer
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-09-06 , DOI: 10.1002/jcp.30023
Lei Xue ₁ , Jun Li ₁ , Yihui Lin ₂ , Degang Liu ₁ , Qiang Yang ₁ , Jinting Jian ₁ , Jiangzhou Peng ₁

Affiliation

N⁶‐methyladenosine (m⁶A) and long noncoding RNAs (lncRNAs) are both crucial regulators in non‐small‐cell lung cancer (NSCLC) tumorigenesis. However, the pathological roles of m⁶A and lncRNAs in NSCLC progression are still limited and undefined. Here, lncRNA ABHD11‐AS1 was upregulated in NSCLC tissue specimens and cells and the ectopic overexpression was closely correlated with unfavorable prognosis of NSCLC patients. Functionally, ABHD11‐AS1 promoted the proliferation and Warburg effect of NSCLC. Mechanistically, m⁶A profile was analyzed by methylated RNA immunoprecipitation sequencing (MeRIP‐Seq). MeRIP‐Seq presented that there was m⁶A modification site in ABHD11‐AS1. m⁶A methyltransferase‐like 3 (METTL3) installed the m⁶A modification and enhanced ABHD11‐AS1 transcript stability to increase its expression. In conclusion, our findings highlight the function and mechanism of METTL3‐induced ABHD11‐AS1 in NSCLC and inspire the understanding of m⁶A and lncRNA in cancer biology.

中文翻译：

m6A 转移酶 METTL3 诱导的 lncRNA ABHD11-AS1 促进非小细胞肺癌的 Warburg 效应

N ⁶甲基腺苷(m ⁶ A) 和长链非编码 RNA (lncRNA) 都是非小细胞肺癌 (NSCLC) 肿瘤发生的关键调节因子。然而，m ⁶ A 和 lncRNA 在 NSCLC 进展中的病理作用仍然有限且未明确。在这里，lncRNA ABHD11-AS1 在 NSCLC 组织标本和细胞中上调，异位过表达与 NSCLC 患者的不良预后密切相关。在功能上，ABHD11-AS1促进了NSCLC的增殖和Warburg效应。从机制上讲，m ⁶ A 谱通过甲基化 RNA 免疫沉淀测序（MeRIP-Seq）进行分析。MeRIP-Seq 表明ABHD11-AS1中存在 m ⁶ A 修饰位点。米⁶甲基转移酶样 3 (METTL3) 安装了 m ⁶ A 修饰并增强了 ABHD11-AS1 转录本稳定性以增加其表达。总之，我们的研究结果突出了 METTL3 诱导的 ABHD11-AS1 在 NSCLC 中的功能和机制，并启发了对 m ⁶ A 和 lncRNA 在癌症生物学中的理解。

更新日期：2020-09-06

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