Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has emerged as a serious threat to human health worldwide. Efficient disinfection of surfaces contaminated with SARS-CoV-2 may help prevent its spread. This study aimed to investigate the in vitro efficacy of 222-nm far-ultraviolet light (UVC) on the disinfection of SARS-CoV-2 surface contamination.

Methods

We investigated the titer of SARS-CoV-2 after UV irradiation (0.1 mW/cm²) at 222 nm for 10-300 seconds using the 50% tissue culture infectious dose (TCID₅₀). In addition, we used quantitative reverse transcription polymerase chain reaction to quantify SARS-CoV-2 RNA under the same conditions.

Results

One and 3 mJ/cm² of 222-nm UVC irradiation (0.1 mW/cm² for 10 and 30 seconds) resulted in 88.5 and 99.7% reduction of viable SARS-CoV-2 based on the TCID₅₀ assay, respectively. In contrast, the copy number of SARS-CoV-2 RNA did not change after UVC irradiation even after a 5-minute irradiation.

Conclusions

This study shows the efficacy of 222-nm UVC irradiation against SARS-CoV-2 contamination in an in vitro experiment. Further evaluation of the safety and efficacy of 222-nm UVC irradiation in reducing the contamination of real-world surfaces and the potential transmission of SARS-CoV-2 is needed.

中文翻译：

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222 nm 紫外线对 SARS-CoV-2 表面污染的消毒效果

背景

导致 2019 年冠状病毒病 (COVID-19) 的严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 已成为全球人类健康的严重威胁。对受 SARS-CoV-2 污染的表面进行有效消毒可能有助于防止其传播。本研究旨在研究 222 nm 远紫外线 (UVC) 对 SARS-CoV-2 表面污染消毒的体外功效。

方法

我们使用 50% 的组织培养感染剂量 (TCID _{50 ) 在 222 nm 的紫外线 (0.1 mW/cm} ² )照射 10-300 秒后研究了 SARS-CoV-2 的滴度。此外，我们使用定量逆转录聚合酶链反应在相同条件下对 SARS-CoV-2 RNA 进行定量。

结果

_{根据 TCID 50}测定，1 和 3 mJ/cm ²的 222 nm UVC 照射（0.1 mW/cm ²持续 10 秒和 30 秒）分别导致存活的 SARS-CoV-2 减少 88.5% 和 99.7% 。相比之下，SARS-CoV-2 RNA 的拷贝数在 UVC 照射后即使照射 5 分钟也没有变化。

结论

这项研究显示了 222 nm UVC 辐射在体外实验中对 SARS-CoV-2 污染的功效。需要进一步评估 222 nm UVC 辐射在减少现实世界表面污染和 SARS-CoV-2 潜在传播方面的安全性和有效性。