The roots of 4 japonica, 4 indica, and 7 hybrid rice varieties were obtained by hydroponic experiment and used to explore the relationship between charge characteristics and exchangeable manganese(II) (Mn(II)) on rice roots and Mn(II) absorption in roots and shoots of the rice. Results indicated Mn(II) adsorbed on rice roots mainly existed as exchangeable Mn(II) after 2 h. The roots of indica and hybrid rice carried more negative charges than the roots of japonica rice. Accordingly, this led to more exchangeable Mn(II) to be adsorbed on roots of indica and hybrid rice after 2 h and more Mn(II) absorbed in the roots of the same varieties after 48 h. However, this was contrary to the result of Mn(II) absorption in rice shoots after 48 h. Coexisting cations of K⁺, Na⁺, Ca²⁺, and Mg²⁺ reduced the exchangeable Mn(II) on rice roots through their competition with Mn(II) for sorption sites on rice roots, which led to the decrease in Mn(II) absorption in rice roots and shoots. Ca²⁺ and Mg²⁺ showed a greater decrease in the Mn(II) absorbed in roots and shoots than K⁺ and Na⁺. The reduction of Mn(II) absorption in the roots of indica rice and hybrid rice induced by Ca²⁺ and Mg²⁺ was more than that of japonica rice. This was attributed to more negative charges on the roots of the former than the latter. Therefore, the absorption of Mn(II) by rice roots was determined by surface charge properties and exchangeable Mn(II) on the rice roots. The results suggested that Ca²⁺ and Mg²⁺ have potential to alleviate Mn(II) toxicity to rice.