Hydrogel has been used in drug delivery systems because of its excellent porosity, biocompatibility, and water retention. However, it remains challenging to medicalize hydrogels and construct an integrated therapeutic system to significantly boost efficiency. Using bovine serum albumin (BSA) and gelatin crosslinked via copper sulfate (Cu²⁺) with a thiol group (-SH), we developed BSA@Gelatin-Cu²⁺-REG, a multifunctional integrally-medicalized hydrogel, as an injectable scaffold loading the functional peptide REGRT (REG), which considerably accelerated vascularization and tissue regeneration. Additionally, Cu²⁺ ions were used as cross-linkers providing antibacterial activity, while the hypoxia-inducible factor (HIF)-1α conferred protection from Cu²⁺ ions and assisted REG in upregulating the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 2 (MMP₂) for angiogenesis and wound healing. Furthermore, the easily biodegradable BSA and gelatin provide an appropriate drug release rate and promote cell adhesion. We determined that BSA@Gelatin-Cu²⁺-REG possessed internal synergy and acted as a thruster for rapid angiogenesis and tissue regeneration, a desired feature in the prognosis of flap transplantation as well as in other surgeries associated with trauma treatment.

由于其优异的孔隙率，生物相容性和保水性，水凝胶已用于药物输送系统。然而，将水凝胶医学化并构建集成的治疗系统以显着提高效率仍然具有挑战性。我们使用牛血清白蛋白（BSA）和通过硫酸铜（Cu ²⁺）与巯基（-SH）交联的明胶，开发了多功能整体药物化水凝胶BSA @ Gelatin-Cu ²⁺ -REG作为可注射支架。加载功能性多肽REGRT（REG），可大大加速血管生成和组织再生。此外，Cu ²⁺离子被用作提供抗菌活性的交联剂，而缺氧诱导因子（HIF）-1α则提供了对Cu ^2+的保护。离子和辅助REG上调血管内皮生长因子（VEGF）和基质金属蛋白酶2（MMP ₂）的表达，从而促进血管生成和伤口愈合。此外，易于生物降解的BSA和明胶可提供适当的药物释放速率并促进细胞粘附。我们确定，BSA @ Gelatin-Cu ²⁺ -REG具有内部协同作用，可作为快速血管生成和组织再生的推进器，这是皮瓣移植以及与创伤治疗相关的其他手术预后中的理想特征。