Self-Assembled Supramolecular Hybrid Hydrogels Based on Host–Guest Interaction: Formation and Application in 3D Cell Culture,ACS Applied Bio Materials

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Self-Assembled Supramolecular Hybrid Hydrogels Based on Host–Guest Interaction: Formation and Application in 3D Cell Culture
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-09-01 , DOI: 10.1021/acsabm.0c00711
Pengfei Ren ₁ , Faming Wang ₁ , Katrien V Bernaerts ₂ , Yifu Fu ₁ , Wanjun Hu ₁ , Naizhen Zhou ₁ , Jidong Dai ₁ , Min Liang ₁ , Tianzhu Zhang ₁

Affiliation

In recent decades, in vitro three-dimensional (3D) cell culture has been rapidly developed and widely used in many biomedical fields. Based on this background, a kind of self-assembled supramolecular hybrid hydrogel materials based on host–guest interaction of β-cyclodextrin (βCD) and adamantane (Ad) is designed for 3D cell culture. First, βCD is grafted to poly(methyl vinyl ether-alt-maleic acid) (PMM) to obtain the host polymers of βCD-grafted-PMM (PMM-βCD). Second, the guest polymers of poly(acrylamide-co-N-adamantyl acrylamide) (PAAm-Ad) are synthesized through free-radical copolymerization of acrylamide and N-adamantyl acrylamide. Finally, the self-assembled supramolecular hybrid hydrogels of PMM-βCD/PAAm-Ad are formed by simply mixing the aqueous solution of host and guest polymers with a total concentration of 3.3% (w/v) and a βCD/Ad molar ratio of 1:1. The main cross-linking interactions come from the host–guest interaction of βCD/Ad as well as hydrogen-bonding interaction of carboxyl/amide groups. The prepared hydrogels with good cytocompatibility have been successfully used as 3D cell culture scaffold for SKOV3, HUVEC, and L929 cells culture. Thus, this work provides a way and biomaterial for the preparation of a functionalized 3D cell culture scaffold, which lays an experimental and theoretical basis for cell follow-up research.

中文翻译：

基于主客体相互作用的自组装超分子杂化水凝胶：在3D细胞培养中的形成和应用

近几十年来，体外三维（3D）细胞培养得到了迅速发展，并广泛应用于许多生物医学领域。基于此背景，设计了一种基于β-环糊精（βCD）和金刚烷（Ad）的主客体相互作用的自组装超分子杂化水凝胶材料，用于3D细胞培养。首先，将βCD接枝到聚（甲基乙烯基醚-alt-马来酸）（PMM）上，得到βCD-接枝-PMM（PMM-βCD）的主体聚合物。其次，通过丙烯酰胺和N的自由基共聚合成了聚（丙烯酰胺-共-N-金刚烷基丙烯酰胺）（PAAm-Ad）的客体聚合物。-金刚烷基丙烯酰胺。最后，通过简单混合总浓度为 3.3% (w/v) 和 βCD/Ad 摩尔比的主体和客体聚合物的水溶液，形成了 PMM-βCD/PAAm-Ad 自组装超分子杂化水凝胶。 1:1。主要的交联相互作用来自βCD/Ad的主客体相互作用以及羧基/酰胺基团的氢键相互作用。制备的具有良好细胞相容性的水凝胶已成功用作 SKOV3、HUVEC 和 L929 细胞培养的 3D 细胞培养支架。因此，该工作为功能化3D细胞培养支架的制备提供了途径和生物材料，为细胞后续研究奠定了实验和理论基础。

更新日期：2020-10-21

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