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Zwitterionic peptide cloak mimics protein surfaces for protein protection.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-08-31 , DOI: 10.1002/anie.202004995
Zhefan Yuan 1 , Bowen Li 2 , Liqian Niu 1 , Chenjue Tang 3 , Patrick McMullen 1 , Priyesh Jain 1 , Yuwei He 1, 4 , Shaoyi Jiang 1, 2
Affiliation  

Inspired by the amino acid composition of natural protein surfaces, we developed a zwitterionic cloak containing multi‐layers of short alternating glutamic acid and lysine (EK) peptides as a facile, highly effective and low‐immunogenicity approach for the protection and delivery of biotherapeutics. Each EK layer grafted to proteins provides multiple times of new lysine reaction sites for the growth of subsequent EK layers. This unique design allows EK peptides to achieve high coating density on proteins, overcoming the limitation of traditional conjugation strategies that rely on the number of innate lysine groups. A triple‐layer EK cloak manifests to successfully eliminate the specific and non‐specific interactions of protected asparaginase with biological media while prolong the drug circulation time and significantly mitigate its immunogenicity in vivo, suggesting an EK peptide cloak as a promising approach to improve the safety and efficacy of biotherapeutics.

中文翻译:

两性离子肽披风可模仿蛋白质表面以提供蛋白质保护。

受天然蛋白质表面氨基酸组成的启发,我们开发了一种两性离子披风,其中包含多层短交替的谷氨酸和赖氨酸(EK)肽,可作为一种简便,高效且低免疫原性的方法来保护和治疗生物治疗剂。嫁接到蛋白质上的每个EK层为后续EK层的生长提供了多次新的赖氨酸反应位点。这种独特的设计使EK肽能够在蛋白质上实现高涂层密度,克服了依赖天然赖氨酸基团数量的传统缀合策略的局限性。
更新日期:2020-08-31
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