DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers.,Nano Letters

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DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers.
Nano Letters ( IF 9.6 ) Pub Date : 2020-08-28 , DOI: 10.1021/acs.nanolett.0c01898
Shixing Chen _{1,

2} , Jing Su _{1,

3} , Zhihan Zhao ₁ , Yuan Shao ₄ , Yanzhi Dou ₁ , Fuwu Li ₅ , Wangping Deng ₁ , Jiye Shi ₁ , Qian Li ₆ , Xiaolei Zuo ₆ , Shiping Song _{1,

5} , Chunhai Fan _{6,

7}

Affiliation

Epigenetic alterations hold great promise as biomarkers for early stage cancer diagnosis. Nevertheless, direct identification of rare methylated DNA in the genome remains challenging. Here, we report an ultrasensitive framework nucleic acid-based electrochemical sensor for quantitative and highly selective analysis of DNA methylation. Notably, we can detect 160 fg of methylated DNA in million-fold unmethylated DNA samples using this electrochemical methylation-specific polymerase chain reaction (E-MSP) method. The high sensitivity of E-MSP enables one-step detection of low-abundance methylation at two different genes in patient serum samples. By using a combination test with two methylation alterations, we achieve high accuracy and sensitivity for reliable differentiation of prostate cancer and benign prostate hypertrophy (BPH). This new method sheds new light on translational use in early cancer diagnosis and in monitoring patients’ responses to therapeutic agents.

中文翻译：

DNA框架支持的前列腺癌DNA甲基化的电化学分析。

表观遗传学改变作为早期癌症诊断的生物标志物具有广阔的前景。然而，直接鉴定基因组中罕见的甲基化DNA仍然具有挑战性。在这里，我们报告基于超灵敏框架核酸的电化学传感器，用于定量和高度选择性地分析DNA甲基化。值得注意的是，使用这种电化学甲基化特异性聚合酶链反应（E-MSP）方法，我们可以在百万倍的未甲基化DNA样品中检测到160 fg甲基化DNA。E-MSP的高灵敏度可以一步检测患者血清样品中两个不同基因的低丰度甲基化。通过使用具有两个甲基化改变的组合测试，我们可以实现对前列腺癌和良性前列腺肥大（BPH）的可靠区分的高精度和灵敏性。

更新日期：2020-10-15

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