Abstract Cellulolytic enzymes produced by Trichoderma sp. have attracted interest in converting the biomass to simple sugars in the production of cellulosic ethanol. In this work, a novel cellulolytic strain M501 was isolated and identified as T. gamsii by sequencing the ITS rDNA region. The production of cellulase (CMCase) by T. gamsii M501 was enhanced by employing statistical methods. The strain grown in the optimized production medium composed of mineral salts, microcrystalline cellulose (13.7 g/l), tryptone (4.8 g/l) and trace elements (2 mL/l) at pH 5.5 and 28 °C for 72 h produced a maximum CMCase of 61.3 U/mL. The optimized production medium also showed the other enzyme activity of FPU (2.6 U/mL), β-glucosidase (2.1 U/mL), xylanase (681 U/mL) and β- xylosidase (0.6 U/mL). The crude cellulase cocktail produced by T. gamsii M501 efficiently hydrolyzed alkali pretreated sugarcane bagasse with glucose and xylose yield of 78 % and 74 % respectively at 10 % solid loading. This study is the first of its kind research on biomass saccharification using T. gamsii cellulase cocktail. Therefore, the novel strain T. gamsii M501 would be useful for further development of an enzyme cocktail for cellulosic ethanol production.

中文翻译：

---

响应面法提高木霉M501新菌株纤维素酶产量及其在生物质糖化中的应用

摘要由木霉属产生的纤维素分解酶。已经引起了人们对在纤维素乙醇生产中将生物质转化为单糖的兴趣。在这项工作中，通过对 ITS rDNA 区域进行测序，分离出一种新的纤维素分解菌株 M501 并鉴定为 T. gamsii。T. gamsii M501 的纤维素酶 (CMCase) 产量通过采用统计方法得到增强。在由矿物盐、微晶纤维素 (13.7 g/l)、胰蛋白胨 (4.8 g/l) 和微量元素 (2 mL/l) 组成的优化生产培养基中在 pH 5.5 和 28 °C 下生长 72 小时的菌株产生了最大 CMCase 为 61.3 U/mL。优化的生产培养基还表现出FPU（2.6 U/mL）、β-葡萄糖苷酶（2.1 U/mL）、木聚糖酶（681 U/mL）和β-木糖苷酶（0.6 U/mL）的其他酶活性。T. 生产的粗纤维素酶混合物。gamsii M501 有效水解碱预处理的甘蔗渣，在 10% 的固体负载下，葡萄糖和木糖的产率分别为 78% 和 74%。本研究是同类研究中首次使用 T. gamsii 纤维素酶混合物进行生物质糖化。因此，新菌株 T. gamsii M501 可用于进一步开发用于纤维素乙醇生产的酶混合物。