基于碳纳米点的荧光纳米探针阵列用于生物多胺的定性和定量测定,Microchimica Acta

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基于碳纳米点的荧光纳米探针阵列用于生物多胺的定性和定量测定
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-08-27 , DOI: 10.1007/s00604-020-04492-4
Na Du ₁ , Zhi Dou ₁ , Yapei Wu ₁ , Qiuhua Wu ₁ , Guolin Zhang ₁ , Xue Liu ₁

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构建了基于荧光富氮碳点 (N-CDs) 和 Ag+ 的纳米探针阵列，用于同时定性和定量测定色胺 (Try)、组胺 (His)、腐胺 (Put) 等七种生物多胺 (BA)。 )、尸胺 (Cad)、精胺 (Spm)、亚精胺 (Spd) 和胍丁胺 (Agm)。Ag+ 可以与 N-CDs 特异性结合并通过静态机制淬灭 N-CDs 的荧光。BA 通过桥接 N-CD@Ag+ 的两个 Ag+ 中心进一步静态猝灭 N-CD@Ag+ 复合材料的荧光。纳米探针阵列是基于由各种 BA 的不同结合亲和力引起的差异荧光响应构建的。BAs 可以通过纳米探针阵列在 0.5-500 μM 的浓度范围内进行区分和分析。初步稀释和人工掺入的商业人血清用于模拟血清环境，以评估纳米探针阵列在实际样品中的性能。N-CD@Ag+ 系统可以在模拟人血清样本中以 100% 的准确率识别 BA。即使在模拟血清环境中，使用 N-CD@Ag+ 系统也实现了 BAs 的定量测定 - 无论是在单组分系统还是在三组分系统中。加标血清样品的回收率高达 99%，相对标准偏差 (RSD) 小于 3%。基于优异的多BA测定性能，构建了BA相关的脑缺血疾病模型。健康病例以及轻度、中度、从基于N-CD@Ag+纳米探针阵列的疾病模型中可以很好地识别出严重的脑缺血病例。由碳纳米点 (N-CD) 和 Ag+ 构建的荧光纳米探针阵列的示意图，用于生物多胺 (BA) 的定性和定量分析以及通过线性判别分析 (LDA) 和支持向量机 (SVM) 诊断脑缺血 (CI)由碳纳米点 (N-CD) 和 Ag+ 构建的荧光纳米探针阵列的示意图，用于生物多胺 (BA) 的定性和定量分析以及通过线性判别分析 (LDA) 和支持向量机 (SVM) 诊断脑缺血 (CI)

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Fluorescent nanoprobe array based on carbon nanodots for qualitative and quantitative determination of biogenic polyamine

A nanoprobe array based on fluorescent nitrogen-rich carbon dots (N-CDs) and Ag+ was constructed for simultaneous qualitative and quantitative determination of seven kinds of biogenic polyamines (BAs), including tryptamine (Try), histamine (His), putrescine (Put), cadaverine (Cad), spermine (Spm), spermidine (Spd), and agmatine (Agm). Ag+ can specifically bind to the N-CDs and quench the fluorescence of the N-CDs through a static mechanism. BAs further statically quench the fluorescence of the N-CD@Ag+ composite by bridging two Ag+ centers of the N-CD@Ag+. The nanoprobe array was constructed based on the differential fluorescence response arising from the differential binding affinity of various BAs. BAs can be differentiated and analyzed by the nanoprobe array within the concentration range 0.5–500 μM. The preliminary diluted and artificially spiked commercial human serum was utilized to simulate the serum environment for assessing the performance of the nanoprobe array in real samples. The N-CD@Ag+ system can recognize BAs with 100% accuracy in simulated human serum samples. The quantitative determination of BAs - no matter in a one-component system or a three-component system - was also realized by using the N-CD@Ag+ system even in the simulated serum environment. The recovery rates from spiked serum samples were higher 99%, and the relative standard deviation (RSD) was less than 3%. Based on the excellent multi-BA determination performance, a BA-related disease model about cerebral ischemia was constructed. Healthy cases as well as mild, moderate, and severe cerebral ischemia cases can be well identified from the disease model based on the N-CD@Ag+ nanoprobe array. Schematic representation of fluorescent nanoprobe array constructed by carbon nanodots (N-CDs) and Ag+ for qualitative and quantitative analyses of biogenic polyamines (BAs) and diagnosis of cerebral ischemia (CI) through linear discriminant analysis (LDA) and support vector machine (SVM) Schematic representation of fluorescent nanoprobe array constructed by carbon nanodots (N-CDs) and Ag+ for qualitative and quantitative analyses of biogenic polyamines (BAs) and diagnosis of cerebral ischemia (CI) through linear discriminant analysis (LDA) and support vector machine (SVM)

更新日期：2020-08-27

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