Multicenter-Emitting Carbon Dots: Color Tunable Fluorescence and Dynamics Monitoring Oxidative Stress In Vivo,Chemistry of Materials

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Multicenter-Emitting Carbon Dots: Color Tunable Fluorescence and Dynamics Monitoring Oxidative Stress In Vivo
Chemistry of Materials ( IF 7.2 ) Pub Date : 2020-08-26 , DOI: 10.1021/acs.chemmater.0c01391
Yi Wang ₁ , Ge Guo ₁ , Jialin Gao ₂ , Zhibin Li ₁ , Xueer Yin ₁ , Changqing Zhu ₁ , Yunsheng Xia ₁

Affiliation

To date, dynamics monitoring oxidative stress on time resolution in vivo has not been achieved due to the limitation of probes, causing the difficulty to comprehensively understand its biological behaviors and functions. Herein, we propose a novel probe, namely highly bright and color tunable “multicenter-emitting” carbon dots (C-dots), for dynamics monitoring oxidative stress in vivo. Combined with experiments and theoretical calculations, we reveal that each C-dot contains multiple conjugated polyaromatic units on their surfaces, which act as fluorescent emitting centers and response sites of hypochlorous acid (HClO) to achieve an instantaneously color-change readout (107 nm, from yellow-green to blue). Accordingly, the proposed C-dots enable the observation of HClO burst and dynamics evolution at fresh wounds of zebrafish larvae. Such a burst occurs only at 1–5 s after cutting, which is an unprecedented thought regarding oxidative stress dynamics. Furthermore, the present study indicates that HClO molecules are principally generated in situ rather than migration.

中文翻译：

多中心发射碳点：颜色可调荧光和动力学监测体内氧化应激。

迄今为止，由于探针的局限性，尚未实现在体内时间分辨率上监测氧化应激的动力学，这导致难以全面了解其生物学行为和功能。在本文中，我们提出了一种新颖的探针，即高亮度和颜色可调的“多中心发射”碳点（C点），用于动态监测体内的氧化应激。结合实验和理论计算，我们发现每个C点在其表面上都包含多个共轭的多芳族单元，它们充当次氯酸（HClO）的荧光发射中心和响应点，以实现瞬时变色读数（107 nm，（从黄绿色到蓝色）。因此，提出的C点能够观察到斑马鱼幼虫新鲜伤口处的HClO爆发和动力学演变。这种破裂仅在切割后的1-5 s发生，这是关于氧化应力动力学的前所未有的想法。此外，本研究表明，HClO分子主要在原位生成，而不是迁移。

更新日期：2020-10-13

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