In this work, novel 5-fluoro-1-methyl/ethyl-1H-indole-2,3-dione 3-[4-(substituted phenyl)-thiosemicarbazones] 6a-n and 7a-n were synthesized. The antiviral effects of the compounds were tested against HSV-1 (KOS), HSV-2 (G) HSV-1 TK^- KOS ACV^r and VV in HEL cell cultures using acyclovir and ganciclovir as standards, and Coxsackie B4 virus in Vero cell cultures using ribavirin and mycophenolic acid as standards. R₂ ethyl substituted 7 derivatives were found effective against viruses tested. R₁ 4-CF₃ substituted 7d, R₁ 4-OCH₃ substituted 7 g and R₁ 3-Cl substituted 7 l showed activity against HSV-1 (KOS), HSV-2 (G) HSV-1 TK^- KOS ACV^r and VV. Whereas only R₁ 4-Br substituted 7n has selective activity against coxsackie B4 virus. Molecular modeling studies of 7d and 7l were performed to determine binding side on HSV-1 glycoprotein B and D, HSV-2 glycoprotein B structures.

在这项工作中，合成了新颖的5-氟-1-甲基/乙基-1 H-吲哚-2,3-二酮3- [4-（取代的苯基）-硫代半脲酮] 6a-n和7a-n。使用阿昔洛韦和更昔洛韦作为标准品，以及在Vero细胞中的柯萨奇B4病毒，测试了该化合物在HEL细胞培养物中针对HSV-1（KOS），HSV-2（G）HSV-1 TK ^- KOS ACV ^r和VV的抗病毒作用利巴韦林和麦考酚酸作为标准品进行培养。发现R ₂乙基取代的7个衍生物对测试的病毒有效。R ₁ 4-CF ₃取代的7d，R ₁ 4-OCH ₃取代的7 g和R ₁ 3-Cl取代的7 l显示出对HSV-1（KOS），HSV-2（G）HSV-1 TK ^- KOS ACV ^r和VV的活性。而只有R ₁ 4-Br取代的7n具有针对柯萨奇B4病毒的选择性活性。进行了7d和7l的分子建模研究，以确定HSV-1糖蛋白B和D，HSV-2糖蛋白B结构的结合侧。