Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots.,Journal of Agricultural and Food Chemistry

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Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-08-23 , DOI: 10.1021/acs.jafc.0c04251
Yun Ma ₁ , Zhi Ma ₁ , Xiyue Huo ₁ , Meiyu Gu ₁ , Sainan Ma ₁ , Yu Jing ₂ , Yixiao Wang ₁ , Ying Yue ₁ , Zhibiao Feng ₃ , Bo Tian ₁

Affiliation

The estimation of yeast viability with B- and N-doped carbon dots (BN-CDs) was investigated in this paper. BN-CDs with a fluorescent quantum yield of 65.47% were prepared by a one-step hydrothermal method. The size distribution of BN-CDs was relatively narrow, with the majority falling within 7.5–8.5 nm, and they were mainly composed of carbon, oxygen, nitrogen, and boron. BN-CDs were shown to have strong and stable fluorescence. They exhibited excitation-independent photoluminescence property, which could avoid the autofluorescence and limitation of the excitation source. Dead and live yeast cells were distinguished well by BN-CD staining in a short time, and there was no strict requirement for light protection. The application of BN-CDs in beer brewing can solve the problem of estimation of yeast viability.

中文翻译：

基于B和N掺杂碳点的酿酒酵母的高效成像。

本文研究了用B和N掺杂碳点（BN-CD）评估酵母活力的方法。通过一步水热法制备了荧光量子产率为65.47％的BN-CD。BN-CD的尺寸分布相对较窄，大多数分布在7.5-8.5 nm之间，并且主要由碳，氧，氮和硼组成。已显示BN-CD具有强而稳定的荧光。它们表现出与激发无关的光致发光特性，可以避免自发荧光和激发源的局限性。BN-CD染色可在短时间内很好地区分死酵母和活酵母细胞，对光防护没有严格要求。BN-CDs在啤酒酿造中的应用可以解决估计酵母活力的问题。

更新日期：2020-09-16

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