Biomaterials Advances ( IF 5.5 ) Pub Date : 2020-08-22 , DOI: 10.1016/j.msec.2020.111393 Silvia A. Ferreira , Gloria Young , Julian R. Jones , Sara Rankin
OssiMend® Bioactive (Collagen Matrix Inc., NJ) is a three-component porous composite bone graft device of 45S5 Bioglass/carbonate apatite/collagen. Our in vitro studies showed that conditioned media of the dissolution products of OssiMend Bioactive stimulated primary human osteoblasts to form mineralized bone-like nodules in vitro in one week, in basal culture media (no osteogenic supplements). Osteoblast differentiation was followed by gene expression analysis and a mineralization assay. In contrast, the dissolution products from commercial OssiMend (Bioglass-free carbonate apatite/collagen scaffolds), or from 45S5 Bioglass particulate alone, did not induce the mineralization of the extracellular matrix, but did induce osteoblast differentiation to mature osteoblasts, evidenced by the strong upregulation of BGLAP and IBSP mRNA levels. The calcium ions and soluble silicon species released from 45S5 Bioglass particles and additional phosphorus release from OssiMend mediated the osteostimulatory effects. Medium conditioned with OssiMend Bioactive dissolution had a much higher concentration of phosphorus and silicon than media conditioned with OssiMend and 45S5 Bioglass alone. While OssiMend and OssiMend Bioactive led to calcium precipitation in cell culture media, OssiMend Bioactive produced a higher concentration of soluble silicon than 45S5 Bioglass and higher dissolution of phosphorus than OssiMend. These in vitro results suggest that adding 45S5 Bioglass to OssiMend produces a synergistic osteostimulation effect on primary human osteoblasts.
In summary, dissolution products of a Bioglass/carbonate apatite/collagen composite scaffold (OssiMend® Bioactive) stimulate human osteoblast differentiation and mineralization of extracellular matrix in vitro without any osteogenic supplements. The mineralization was faster than for dissolution products of ordinary Bioglass.
中文翻译:
生物玻璃/碳酸盐磷灰石/胶原蛋白复合支架溶解产物促进人成骨细胞分化
OssiMend®Bioactive(Collagen Matrix Inc.,NJ)是45S5 Bioglass /碳酸盐磷灰石/胶原蛋白的三组分多孔复合骨移植装置。我们的体外研究表明,生物活性OssiMend的溶解产品的条件培养基刺激的初级人成骨细胞形成矿化骨样结节体外在一周内,在基础培养基中(无成骨补充剂)。成骨细胞分化后进行基因表达分析和矿化测定。相比之下,商品化的OssiMend(不含生物玻璃的碳酸盐磷灰石/胶原蛋白支架)或单独来自45S5生物玻璃的颗粒的溶出产物不会诱导细胞外基质的矿化,但会诱导成骨细胞分化为成熟的成骨细胞,这一点证明了BGLAP和IBSP的上调mRNA水平。从45S5生物玻璃颗粒释放的钙离子和可溶性硅物质以及从OssiMend释放的其他磷介导了骨刺激作用。与仅使用OssiMend和45S5 Bioglass进行处理的介质相比,使用OssiMend生物活性溶解进行处理的介质的磷和硅浓度要高得多。虽然OssiMend和OssiMend Bioactive导致钙在细胞培养基中沉淀,但OssiMend Bioactive产生的可溶性硅浓度比45S5 Bioglass高,而磷的溶解度高于OssiMend。这些体外结果表明,将45S5生物玻璃添加到OssiMend中可对原代人成骨细胞产生协同的骨刺激作用。
总之,无需任何成骨补充剂,生物玻璃/碳磷灰石/胶原蛋白复合支架的溶解产物(OssiMend®Bioactive)在体外刺激人成骨细胞分化和细胞外基质矿化。矿化比普通生物玻璃的溶解产物要快。