PDMPO：特定的硅或二氧化硅、pH 敏感荧光探针？,RSC Advances

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PDMPO：特定的硅或二氧化硅、pH 敏感荧光探针？
RSC Advances ( IF 3.9 ) Pub Date : 2020-8-21 , DOI: 10.1039/d0ra05108j
Patricia Merdy ₁ , Cyril Neytard ₁ , Jean-Dominique Meunier ₂ , Yves Lucas ₁

Affiliation

为了了解土壤和植物中的 Si 行为和生物可分配性，我们评估了PDMPO (2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole) 的使用，它被认为是Si 特异性荧光标记并具有 pH 依赖性荧光。我们研究了PDMPO与水溶性 Si、Al 和天然有机物（腐植酸，HA）之间的相互作用。通过荧光光谱在 pH 4、7 和 9 下研究了六种不同 HA、Si 和 Al 浓度的体系。Al- PDMPO复合物通过红外光谱表征，溶液中的粒径分布通过纳米跟踪分析表征。我们发现，当通常的 pH 缓冲液不存在时，PDMPO荧光不依赖于 pH 值，也不是 Si 特异性的。在PDMPO -Si-HA 体系中，PDMPO荧光信号大大增强，表明形成了高荧光三元 HA -PDMPO- Si 基团。当向系统中添加 Al 时，荧光被强烈猝灭，表明形成了低荧光的四元 HA - PDMPO -Si-Al 基团。因此，PDMPO荧光对可络合金属和天然有机物非常敏感，因此难以应用于复杂介质中 Si 或 pH 值的定量。

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PDMPO: a specific silicon or silica, pH sensitive fluorescent probe?

In order to understand Si behavior and biodisponibility in soils and plants, we evaluated the use of PDMPO (2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole) that was supposed to be a Si-specific fluorescence marker and to have a pH-dependent fluorescence. We studied the interactions between PDMPO and water-dissolved Si, Al and natural organic matter (humic acids, HA). Six systems with different HA, Si and Al concentrations were studied by fluorescence spectroscopy at pH 4, 7 and 9. The Al–PDMPO complex was characterized by infrared spectroscopy and the particle size distribution in solution was characterized by nano tracking analysis. We found that when usual pH buffers are not present, the PDMPO fluorescence was not pH dependent and was not Si-specific. In the PDMPO–Si–HA system, the PDMPO fluorescence signals were greatly enhanced, suggesting the formation of highly fluorescent ternary HA–PDMPO–Si groups. When Al was added to the system, the fluorescence was strongly quenched, suggesting the formation of low-fluorescence quaternary HA–PDMPO–Si–Al groups. The PDMPO fluorescence is therefore greatly sensitive to complexable metals and to natural organic matter and is therefore difficult to be applied for the quantification of Si or pH in a complex medium.

更新日期：2020-08-21

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