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NADH-Activated Dual-Channel Fluorescent Probes for Multicolor Labeling of Live Cells and Tumor Mimic Spheroids.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-08-19 , DOI: 10.1021/acs.analchem.0c02049 Arup Podder 1, 2 , Vishnu Priya Murali 3 , Selvakumar Deepika 3 , Avinash Dhamija 4 , Shayeri Biswas 1 , Kaustabh K Maiti 3 , Sankarprasad Bhuniya 1, 5
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-08-19 , DOI: 10.1021/acs.analchem.0c02049 Arup Podder 1, 2 , Vishnu Priya Murali 3 , Selvakumar Deepika 3 , Avinash Dhamija 4 , Shayeri Biswas 1 , Kaustabh K Maiti 3 , Sankarprasad Bhuniya 1, 5
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The 1,4-dihydronicotinamide adenine dinucleotide (NADH) is one of the key coenzymes that participates in various metabolic processes including maintaining the redox balance. Early information on the imbalance of NADH is crucial in the context of diagnosing the pathogenic conditions. Thus, a dual-channel fluorescent probe (MQN) is developed for tracking of NADH/NAD(P)H in live cells. In the presence of NADH, only it showed emission signals at 460 and 550 nm upon excitation at 390 and 450 nm, respectively. The probe could provide accurate information on NADH levels in cancer cells (HeLa) and normal cells (WI-38). We observed that the NADH level in cancer cells (HeLa) is relatively higher than that in normal WI-38 cells. We received similar information on NADH upon calibrating with a commercial NADH kit. Moreover, we evaluated substrate-specific NADH expression in the glycolysis pathway and oxidative phosphorylation process. Also, the dual-channel probe MQN has visualized NADH manipulation in the course of depletion of GSH to maintain cellular redox balance. This dual-channel molecular probe MQN comes out as a new detection tool for NADH levels in live cells and tumor mimic spheroids.
中文翻译：
NADH激活的双通道荧光探针，用于活细胞和肿瘤模拟球体的多色标记。
1,4-二氢烟酰胺酰胺腺嘌呤二核苷酸（NADH）是参与各种代谢过程（包括维持氧化还原平衡）的关键辅酶之一。关于NADH失衡的早期信息对于诊断病原体至关重要。因此，双通道荧光探针（MQN（）用于追踪活细胞中的NADH / NAD（P）H。在存在NADH的情况下，只有在分别在390和450 nm激发时才显示460和550 nm的发射信号。该探针可以提供有关癌细胞（HeLa）和正常细胞（WI-38）中NADH水平的准确信息。我们观察到癌细胞（HeLa）中的NADH水平相对高于正常WI-38细胞中的NADH水平。使用商用NADH套件进行校准后，我们收到了有关NADH的类似信息。此外，我们评估了糖酵解途径和氧化磷酸化过程中底物特异性NADH的表达。同样，双通道探针MQN在消耗GSH的过程中可视化了NADH操纵，以维持细胞氧化还原平衡。这款双通道分子探针MQN 作为活细胞和肿瘤模拟球体中NADH水平的新检测工具而出现。
更新日期：2020-09-15
中文翻译：
NADH激活的双通道荧光探针，用于活细胞和肿瘤模拟球体的多色标记。
1,4-二氢烟酰胺酰胺腺嘌呤二核苷酸（NADH）是参与各种代谢过程（包括维持氧化还原平衡）的关键辅酶之一。关于NADH失衡的早期信息对于诊断病原体至关重要。因此，双通道荧光探针（MQN（）用于追踪活细胞中的NADH / NAD（P）H。在存在NADH的情况下，只有在分别在390和450 nm激发时才显示460和550 nm的发射信号。该探针可以提供有关癌细胞（HeLa）和正常细胞（WI-38）中NADH水平的准确信息。我们观察到癌细胞（HeLa）中的NADH水平相对高于正常WI-38细胞中的NADH水平。使用商用NADH套件进行校准后，我们收到了有关NADH的类似信息。此外，我们评估了糖酵解途径和氧化磷酸化过程中底物特异性NADH的表达。同样，双通道探针MQN在消耗GSH的过程中可视化了NADH操纵，以维持细胞氧化还原平衡。这款双通道分子探针MQN 作为活细胞和肿瘤模拟球体中NADH水平的新检测工具而出现。
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