A Highly Sensitive Catalytic Hairpin Assembly-Based Dynamic Light-Scattering Biosensors for Telomerase Detection in Bladder Cancer Diagnosis.,Analytical Chemistry

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A Highly Sensitive Catalytic Hairpin Assembly-Based Dynamic Light-Scattering Biosensors for Telomerase Detection in Bladder Cancer Diagnosis.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-08-19 , DOI: 10.1021/acs.analchem.0c02858
Li Zou ₁ , Xinghui Li ₂ , Ji Zhang ₃ , Liansheng Ling ₄

Affiliation

Precise evaluation of telomerase activity is highly crucial for early cancer diagnosis. In this study, a sensitive catalytic hairpin assembly–dynamic light scattering (CHA–DLS) assay for telomerase activity detection is developed by using the diameter change of gold nanoparticle (AuNP) probes. The telomerase substrate primer can be extended in the presence of telomerase, producing a telomerase extension product (TEP) with telomeric repeat units (TTAGGG)_n at its 3′-end. The TEP can specifically trigger the CHA process and form tremendous AuNPs-H1/H2 nanostructures, resulting in a significant increase in the diameter measured by DLS. Telomerase activity from different cancer cell lines (MCF-7, Huh7, and 5637) was detected using the proposed strategy, the diameter of AuNP probes increased with the number of cancer cells, and this method can accurately detect telomerase activity down to 6 MCF-7 cells, 10 Huh7 cells, and three 5637 cells. Moreover, the CHA–DLS biosensor was successfully applied in urine specimens from healthy individuals and different cancer patients, which can distinguish bladder cancer patients from healthy people and other cancer patients, indicating that the noninvasive method has a great potential for application in early diagnosis of bladder cancer.

中文翻译：

一种基于高灵敏度催化发夹装配的动态光散射生物传感器，用于膀胱癌诊断中的端粒酶检测。

端粒酶活性的准确评估对于早期癌症诊断至关重要。在这项研究中，通过使用金纳米颗粒（AuNP）探针的直径变化，开发了一种用于端粒酶活性检测的灵敏催化发夹组装-动态光散射（CHA–DLS）分析。端粒酶衬底引物可在端粒酶的存在下延伸，产生一端粒酶延伸产物（TEP）与端粒重复单元（TTAGGG）_ñ在其3'端。TEP可以特异性触发CHA过程并形成巨大的AuNPs-H1 / H2纳米结构，从而导致DLS测量的直径显着增加。使用提出的策略检测了来自不同癌细胞系（MCF-7，Huh7和5637）的端粒酶活性，AuNP探针的直径随癌细胞数量的增加而增加，这种方法可以准确地检测低至6 MCF-的端粒酶活性7个细胞，10个Huh7细胞和3个5637细胞。此外，CHA-DLS生物传感器已成功应用于健康个体和不同癌症患者的尿液标本中，可以将膀胱癌患者与健康人和其他癌症患者区分开来，这表明无创方法在早期诊断糖尿病中具有巨大的应用潜力。膀胱癌。

更新日期：2020-09-15

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