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Characterization of Free Fatty Acid Receptor 4 and Its Involvement in Nutritional Control and Immune Response in Pacific Oysters (Crassostrea gigas).
ACS Omega ( IF 3.7 ) Pub Date : 2020-08-19 , DOI: 10.1021/acsomega.0c01325
Busu Li 1, 2, 3 , Li Li 1, 3, 4, 5 , Wei Wang 1, 3 , Jie Meng 1, 3 , Fei Xu 1, 3, 4 , Fucun Wu 1, 2, 6 , Guofan Zhang 1, 2, 3, 4
ACS Omega ( IF 3.7 ) Pub Date : 2020-08-19 , DOI: 10.1021/acsomega.0c01325
Busu Li 1, 2, 3 , Li Li 1, 3, 4, 5 , Wei Wang 1, 3 , Jie Meng 1, 3 , Fei Xu 1, 3, 4 , Fucun Wu 1, 2, 6 , Guofan Zhang 1, 2, 3, 4
Affiliation
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Free fatty acid receptor 4 (FFAR4) has various physiological functions, including energy regulation and immunological homeostasis. We examined the only FFAR4 homologue in the Pacific oyster Crassostrea gigas (CgFFAR4), which functions as a sensor of long-chain fatty acids. CgFFAR4 is 1098 bp long and contains a seven-transmembrane G protein-coupled receptor domain. CgFFAR4 expression was high in the hepatopancreas, but it was downregulated after fasting, indicating that it plays an essential role in food digestion. Lipopolysaccharide stimulation downregulated CgFFAR4 level, probably as an immune response of the animal. Reduced glycogen level alongside decreased insulin receptor, insulin receptor substrate, and C. gigas glycogen synthase transcription levels after CgFFAR4 knockdown revealed that CgFFAR4 was involved in the regulation of fatty acid and glycogen levels via the insulin pathway. Accordingly, this is the first study on an invertebrate FFAR and provides new insights into the role of this receptor in immune response and nutritional control.
中文翻译：
游离脂肪酸受体4的表征及其在太平洋牡蛎（Crassostrea gigas）中的营养控制和免疫反应中。
游离脂肪酸受体4（FFAR4）具有多种生理功能，包括能量调节和免疫平衡。我们检查了太平洋牡蛎Crassostrea gigas（CgFFAR4）中唯一的FFAR4同源物，其功能是长链脂肪酸的传感器。CgFFAR4长1098 bp，包含一个七跨膜G蛋白偶联的受体域。CgFFAR4在肝胰腺中的表达较高，但在禁食后却被下调，表明它在食物消化中起着至关重要的作用。脂多糖刺激下调CgFFAR4水平，可能是动物的免疫反应。CgFFAR4敲低后，糖原水平降低，胰岛素受体，胰岛素受体底物和C. gigas糖原合酶转录水平降低，这表明CgFFAR4通过胰岛素途径参与脂肪酸和糖原水平的调节。因此，这是对无脊椎动物FFAR的首次研究，并提供了对该受体在免疫应答和营养控制中的作用的新见解。
更新日期：2020-09-01
中文翻译：
游离脂肪酸受体4的表征及其在太平洋牡蛎（Crassostrea gigas）中的营养控制和免疫反应中。
游离脂肪酸受体4（FFAR4）具有多种生理功能，包括能量调节和免疫平衡。我们检查了太平洋牡蛎Crassostrea gigas（CgFFAR4）中唯一的FFAR4同源物，其功能是长链脂肪酸的传感器。CgFFAR4长1098 bp，包含一个七跨膜G蛋白偶联的受体域。CgFFAR4在肝胰腺中的表达较高，但在禁食后却被下调，表明它在食物消化中起着至关重要的作用。脂多糖刺激下调CgFFAR4水平，可能是动物的免疫反应。CgFFAR4敲低后，糖原水平降低，胰岛素受体，胰岛素受体底物和C. gigas糖原合酶转录水平降低，这表明CgFFAR4通过胰岛素途径参与脂肪酸和糖原水平的调节。因此，这是对无脊椎动物FFAR的首次研究，并提供了对该受体在免疫应答和营养控制中的作用的新见解。
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