In this paper, a photoinduced electron transfer (PET)& twisted intramolecular charge transfer (TICT)-based fluorescent probe (1) for detecting biothiols (GSH/Cys/Hcy) and hydrogen sulfide with fluorescence turn on was developed. The probe could recognize hydrogen sulfide over primary ions and selectively detect GSH/Cys/Hcy over other amino acids with fluorescence turn-on (an ESIPT process). H₂S can be distinguished from GSH/Cys/Hcy with wavelength shift by UV–Vis spectra. In addition, detection limits for H₂S/GSH/Cys/Hcy of probe 1 were 1.42 μM (0–100 μM), 0.13 μM (0–40 μM), 0.27 (0–30 μM), 0.22 μM (0–40 μM), respectively. The proposed thiolysis of the 2,4-dinitrochlorophenyl ether reaction in identification process was verified by the characteristic peak in ¹H NMR and HRMS spectra. Finally, the biological imaging experiments and low cytotoxicity investigations in HeLa cells demonstrated that probe 1 could provide a promising method for the determination of H₂S and biothiols in aqueous solution and living cells.

本文开发了一种基于光诱导电子转移（PET）和扭曲分子内电荷转移（TICT）的荧光探针（1），用于在开启荧光的情况下检测生物硫醇（GSH / Cys / Hcy）和硫化氢。该探针可以识别初级离子上的硫化氢，并通过荧光开启（ESIPT过程）选择性检测其他氨基酸上的GSH / Cys / Hcy。H ₂ S可以通过UV-Vis光谱的波长偏移与GSH / Cys / Hcy区分。此外，探针1的H ₂ S / GSH / Cys / Hcy的检出限分别为1.42μM（0-100μM），0.13μM（0-40μM），0.27（0-30μM），0.22μM（0-40μM）。通过¹ H NMR和HRMS光谱中的特征峰验证了在鉴定过程中拟议的2,4-二硝基氯苯基醚反应的硫解作用。最后，HeLa细胞的生物学成像实验和低细胞毒性研究表明，探针1可以为测定水溶液和活细胞中的H ₂ S和生物硫醇提供有希望的方法。