变形虫基因组揭示了宿主对质体内共生的主要贡献。,Molecular Biology and Evolution

当前位置： X-MOL 学术 › Mol. Biol. Evol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

变形虫基因组揭示了宿主对质体内共生的主要贡献。
Molecular Biology and Evolution ( IF 11.0 ) Pub Date : 2020-08-13 , DOI: 10.1093/molbev/msaa206
Duckhyun Lhee ₁ , JunMo Lee ₂ , Khaoula Ettahi ₁ , Chung Hyun Cho ₁ , Ji-San Ha ₁ , Ya-Fan Chan ₃ , Udi Zelzion ₃ , Timothy G Stephens ₃ , Dana C Price ₄ , Arwa Gabr ₅ , Eva C M Nowack ₆ , Debashish Bhattacharya ₃ , Hwan Su Yoon ₁

Affiliation

真核生物光合作用细胞质体是许多水生和陆地生态系统的强大动力。藻类和植物中的规范质体起源于十亿多年前，因此对于细胞器进化的初始阶段提供的见解有限。为了解决这个问题，我们在此集中讨论经历了最近一次（约124 Mya）原发性内共生作用的光合变形虫变形虫Paulinella微孔菌菌株KR01（以下称KR01），从而产生了一种称为色团的光合细胞器。对基因组和转录组数据的分析产生了707 Mbp大小的高质量草稿集和32,361个预测基因模型。在计算机上预测总共有291种以色谱为目标的蛋白质，其中206种包含光合作用中的祖细胞器蛋白质组。Paulinella种在核苷酸代谢和氧化应激反应等方面具有功能。基因共表达分析确定了包含已知的高光应激反应基因以及各种功能未知的基因（“暗”基因）的网络。我们鉴定了该物种昼夜节律性基因的特征，发现超过51％的是深色。最近有假设认为，大的双链DNA病毒可能已将基因转移到Paulinella的核中并促进了共生。我们的分析不支持这种想法，而是暗示KR01和紧密相关的微孔假单胞菌MYN1基因组中的这些病毒是最近的入侵所致。

"点击查看英文标题和摘要"

Amoeba genome reveals dominant host contribution to plastid endosymbiosis.

Eukaryotic photosynthetic organelles, plastids, are the powerhouses of many aquatic and terrestrial ecosystems. The canonical plastid in algae and plants originated >1 billion years ago and therefore offers limited insights into the initial stages of organelle evolution. To address this issue, we focus here on the photosynthetic amoeba Paulinella micropora strain KR01 (hereafter, KR01) that underwent a more recent (ca. 124 Mya) primary endosymbiosis, resulting in a photosynthetic organelle termed the chromatophore. Analysis of genomic and transcriptomic data resulted in a high-quality draft assembly of size 707 Mbp and 32,361 predicted gene models. A total of 291 chromatophore targeted proteins were predicted in silico, 206 of which comprise the ancestral organelle proteome in photosynthetic Paulinella species with functions, among others, in nucleotide metabolism and oxidative stress response. Gene co-expression analysis identified networks containing known high light stress response genes as well as a variety of genes of unknown function (“dark” genes). We characterized diurnally rhythmic genes in this species and found that over 51% are dark. It was recently hypothesized that large double-stranded DNA viruses may have driven gene transfer to the nucleus in Paulinella and facilitated endosymbiosis. Our analyses do not support this idea, but rather suggest that these viruses in the KR01 and closely related P. micropora MYN1 genomes resulted from a more recent invasion.

更新日期：2020-08-14

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南