Abstract Urchin-like trimanganese tetroxide (Mn3O4) particles were synthesized through a simple and gentle method, and characterized by XRD, XPS, FTIR, SEM, TEM, and UV–vis absorption. The intrinsic enzyme-like activity of urchin-like Mn3O4 particles was evaluated by 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the absence of H2O2. The optimization of experiment conditions of urchin-like Mn3O4 particles on the oxidase-like activity was further investigated. It was exhibited that urchin-like Mn3O4 particles were best-performing with a highly oxidase-like catalytic activity in the model reaction. Due to the selective inhibition of glutathione (GSH) on the high oxidase-like activity of urchin-like Mn3O4 particles, a selective and sensitive colorimetric assay for GSH had been established. The method exhibited linear relationship over the GSH concentration range from 0.1 μM to 30 μM, with a low detection limit of 20.0 nM and a rapid response of less than 3 min. Based on high specificity of GSH inhibition, urchin-like Mn3O4 particles could be used to detect GSH concentration in human serum samples.

中文翻译：

---

具有氧化酶样活性的海胆样四氧化三锰颗粒用于谷胱甘肽检测

摘要 通过一种简单温和的方法合成了类海胆四氧化三锰 (Mn3O4) 颗粒，并通过 XRD、XPS、FTIR、SEM、TEM 和 UV-vis 吸收进行表征。在不存在 H2O2 的情况下，通过 3, 3', 5, 5'-四甲基联苯胺 (TMB) 评估海胆状 Mn3O4 颗粒的内在酶样活性。进一步研究了类海胆 Mn3O4 颗粒对类氧化酶活性实验条件的优化。结果表明，海胆状 Mn3O4 颗粒在模型反应中表现最佳，具有高度氧化酶样催化活性。由于谷胱甘肽 (GSH) 对海胆状 Mn3O4 颗粒的高氧化酶样活性有选择性抑制作用，因此已经建立了一种选择性和灵敏的 GSH 比色法。该方法在 0.1 μM 至 30 μM 的 GSH 浓度范围内表现出线性关系，检测下限为 20.0 nM，快速响应不到 3 分钟。基于GSH抑制的高度特异性，海胆状Mn3O4颗粒可用于检测人血清样品中的GSH浓度。